Nanoprobes: Quantitatively Detecting the Femtogram Level of Arsenite Ions in Live Cells

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• 作者：Ru Liu ; Zhong Chen ; Yaling Wang ; Yanyan Cui ; Huarui Zhu ; Ping Huang ; Wei Li ; Yuliang Zhao ; Ye Tao ; Xueyun Gao
• 刊名：ACS Nano
• 出版年：2011
• 出版时间：July 26, 2011
• 年：2011
• 卷：5
• 期：7
• 页码：5560-5565
• 全文大小：862K
• 年卷期：0
• ISSN：1936-086X

文摘

In this report, nanoprobes which could detect the femtogram level of arsenite ions in subcellular organelle of live cells are disclosed. The nanoprobes are composed of ssDNA and single-wall carbon nanotubes (SWCNTs), and the ssDNA is marked by a dye molecule. In a live cell, trace arsenite ions could interact with nanoprobes and significantly decrease the emission of the nanoprobes. With the help of a confocal microscope and cryo-electron microscopy, the lysosome target of the arsenite ion and nanoprobe is well described in high spatial resolution.