FRET-Based Mito-Specific Fluorescent Probe for Ratiometric Detection and Imaging of Endogenous Peroxynitrite: Dyad of Cy3 and Cy5

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• 作者：Xiaotong Jia ; Qiangqiang Chen ; Yingfang Yang ; Yao Tang ; Rui Wang ; Yufang Xu ; Weiping Zhu ; Xuhong Qian
• 刊名：Journal of the American Chemical Society
• 出版年：2016
• 出版时间：August 31, 2016
• 年：2016
• 卷：138
• 期：34
• 页码：10778-10781
• 全文大小：347K
• 年卷期：0
• ISSN：1520-5126

文摘

Peroxynitrite (OONO^–) is profoundly implicated in health and disease. The physiological and pathological outcome of OONO^– is related to its local concentration, and hence, a reliable OONO^– assay is highly desired. We have developed a FRET-based small-molecule fluorescent probe (PNCy3Cy5), harnessing the differential reactivity of Cy3 and Cy5 toward OONO^– by fine-tuning. It exhibits high detection sensitivity and yields a ratiometric fluorescent signal. We have exemplified that it can be applied in semiquantitative determination of OONO^– in living cells. Notably, it specifically localizes in mitochondria, where endogenous OONO^– is predominantly generated. Thus, PNCy3Cy5 is a promising molecular tool for peroxynitrite biology.