Magnesium Ion Selective Two-Photon Fluorescent Probe Based on a Benzo[h]chromene Derivative for in Vivo Imaging

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• 作者：Hwan Myung Kim ; Pil Rye Yang ; Mun Sik Seo ; Jae-Sung Yi ; Jin Hee Hong ; Seung-Joon Jeon ; Young-Gyu Ko ; Kyoung J. Lee ; Bong Rae Cho
• 刊名：Journal of Organic Chemistry
• 出版年：2007
• 出版时间：March 16, 2007
• 年：2007
• 卷：72
• 期：6
• 页码：2088 - 2096
• 全文大小：726K
• 年卷期：v.72,no.6(March 16, 2007)
• ISSN：1520-6904

文摘

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A novel, two-photon probe for the detection of free Mg²⁺ ions in living cells and live tissues has beendeveloped. The probe can be excited by 880 nm laser photons, emits strong two-photon excited fluorescencein response to Mg²⁺ ions, can be easily loaded into the cell and tissue, shows high photostability, andcan measure the Mg²⁺ ion concentration without interference by Ca²⁺ ions in living cells. The intracellulardissociation constant (K_dⁱ) for Mg²⁺ determined by the two-photon process is 2.5 mM, which is suitablefor dynamic Mg²⁺ concentration measurement. In addition, the probe is capable of imaging endogenousstores of free Mg²⁺ at a few hundred micrometers depth in live tissues using two-photon microscopy(TPM).