Effective Antisense Gene Regulation via Noncationic, Polyethylene Glycol Brushes

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• 作者：Xueguang Lu ; Fei Jia ; Xuyu Tan ; Dali Wang ; Xueyan Cao ; Jiamin Zheng ; Ke Zhang
• 刊名：Journal of the American Chemical Society
• 出版年：2016
• 出版时间：July 27, 2016
• 年：2016
• 卷：138
• 期：29
• 页码：9097-9100
• 全文大小：356K
• 年卷期：0
• ISSN：1520-5126

文摘

Negatively charged nucleic acids are often complexed with polycationic transfection agents before delivery. Herein, we demonstrate that a noncationic, biocompatible polymer, polyethylene glycol, can be used as a transfection vector by forming a brush polymer-DNA conjugate. The brush architecture provides embedded DNA strands with enhanced nuclease stability and improved cell uptake. Because of the biologically benign nature of the polymer component, no cytotoxicity was observed. This approach has the potential to address several long-lasting challenges in oligonucleotide therapeutics.