Polymerase Chain Reaction on a Viral Nanoparticle

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• 作者：James Carr-Smith ; Ra煤l Pacheco-G贸mez ; Haydn A. Little ; Matthew R. Hicks ; Sandeep Sandhu ; Nadja Steinke ; David J. Smith ; Alison Rodger ; Sarah A. Goodchild ; Roman A. Lukaszewski ; James. H. R. Tucker ; Timothy R. Dafforn
• 刊名：ACS Synthetic Biology
• 出版年：2015
• 出版时间：December 18, 2015
• 年：2015
• 卷：4
• 期：12
• 页码：1316-1325
• 全文大小：498K
• ISSN：2161-5063

文摘

The field of synthetic biology includes studies that aim to develop new materials and devices from biomolecules. In recent years, much work has been carried out using a range of biomolecular chassis including 伪-helical coiled coils, 尾-sheet amyloids and even viral particles. In this work, we show how hybrid bionanoparticles can be produced from a viral M13 bacteriophage scaffold through conjugation with DNA primers that can template a polymerase chain reaction (PCR). This unprecedented example of a PCR on a virus particle has been studied by flow aligned linear dichroism spectroscopy, which gives information on the structure of the product as well as a new protototype methodology for DNA detection. We propose that this demonstration of PCR on the surface of a bionanoparticle is a useful addition to ways in which hybrid assemblies may be constructed using synthetic biology.