PEGylated Lipoplexes: Preparation Protocols Affecting DNA Condensation and Cell Transfection Efficiency

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• 作者：Cecilia Bombelli ; Francesca Faggioli ; Paola Luciani ; Giovanna Mancini ; Maria Grazia Sacco
• 刊名：Journal of Medicinal Chemistry
• 出版年：2007
• 出版时间：November 29, 2007
• 年：2007
• 卷：50
• 期：24
• 页码：6274 - 6278
• 全文大小：200K
• 年卷期：v.50,no.24(November 29, 2007)
• ISSN：1520-4804

文摘

The inclusion of poly(ethylene glycol) monolaurate in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and certain cationic gemini surfactants improves their capability of condensing DNAinto a phase and transfecting it into cells. Both the condensation, observed by circular dichroism, and thetransfection efficiency are strongly effected by the protocol of inclusion of the polymer in the formulations.The highest transfection efficiency is observed in correspondence of the highest extent of DNA condensation.