Poly-3-hydroxyoctanoate P(3HO), a Medium Chain Length Polyhydroxyalkanoate Homopolymer from Pseudomonas mendocina

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• 作者：Ranjana Rai ; Darmawati M. Yunos ; Aldo R. Boccaccini ; Jonathan C. Knowles ; Ian A. Barker ; Steven M. Howdle ; Gregory D. Tredwell ; Tajalli Keshavarz ; Ipsita Roy
• 刊名：Biomacromolecules
• 出版年：2011
• 出版时间：June 13, 2011
• 年：2011
• 卷：12
• 期：6
• 页码：2126-2136
• 全文大小：973K
• 年卷期：v.12,no.6(June 13, 2011)
• ISSN：1526-4602

文摘

Pseudomonas mendocina was found to produce a unique homopolymer of poly(3-hydroxyoctanoate), P(3HO), rather than a copolymer, when grown on sodium octanoate as the sole carbon source. Although this polymer has been produced by other organisms, interestingly this is the first time an absolute homopolymer has been produced by a wild type organism. In addition, a detailed study on the effects of different extraction methods on the yield, molecular weight, thermal properties, and lipopolysaccharide content of P(3HO) has been carried out. The organism was able to accumulate P(3HO) up to 31.38% of its dry cell weight within 48 h in mineral salt medium. Characterization of the monomer was carried out using FTIR, GC-MS, ¹³C, ¹H, and HSQC NMR spectroscopy. The polymer had a crystallinity of 37.5%, Young鈥檚 modulus value of 11.6 MPa and contact angle of 77.3掳. Microstructural studies of solvent cast polymer films revealed a smooth surface topography with a root-mean-square roughness value of 0.238 渭m.