Evaluation of Microbeads of Calcium Alginate as a Fluidized Bed Medium for Affinity Chromatography of Aspergillus niger Pectinase

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• 作者：Ipsita Roy ; Sulakshana Jain ; Sunita Teotia ; and Munishwar Nath Gupta
• 刊名：Biotechnology Progress
• 出版年：2004
• 出版时间：October 2004
• 年：2004
• 卷：20
• 期：5
• 页码：1490 - 1495
• 全文大小：203K
• 年卷期：v.20,no.5(October 2004)
• ISSN：1520-6033

文摘

Calcium alginate microbeads (212-425 m) were prepared by spraying 2% (w/v)alginate solution into 1 M CaCl₂ solution. The fluidization behavior of these beadswas studied, and the bed expansion index and terminal velocity were found to be 4.3and 1808 cm h^-1, respectively. Residence time distribution curves showed that thedispersion of the protein was much less with these microbeads than with conventionallyprepared calcium alginate macrobeads when both kinds of beads were used forchromatography in a fluidized bed format. The fluidized bed of these beads was usedfor the purification of pectinase from a commercial preparation. The media performedwell even with diluted feedstock; 90% activity recovery with 211-fold purification wasobserved.