文摘
Biotransformation of PFOS-precursors (PreFOS) may contribute significantly to the level of perfluorooctanesulfonate (PFOS) in the environment. Perfluorooctane sulfonamide (PFOSA) is one of the major intermediates of higher molecular weight PreFOS. Its further degradation to PFOS could be isomer specific and thereby explain unexpected high percentages of branched (Br-) PFOS isomers observed in wildlife. In this study, isomeric degradation of PFOSA was concomitantly investigated by in vivo and in vitro tests using common carp as an animal model. In the in vivo tests branched isomers of PFOSA and PFOS were eliminated faster than the corresponding linear (n-) isomers, leading to enrichment of n-PFOSA in the fish. In contrast, Br-PFOS was enriched in the fish, suggesting that Br-PFOSA isomers were preferentially metabolized to Br-PFOS over n-PFOSA. This was confirmed by the in vitro test. The exception was 1m-PFOSA, which could be the most difficult to be metabolized due to its 伪-branched structure, resulting in the deficiency of 1m-PFOS in the fish. The in vitro tests indicated that the metabolism mainly took place in the fish liver instead of its kidney, and it was mainly a Phase I reaction. The results may help to explain the special PFOS isomer profile observed in wildlife.
