Glycomic Analysis of Membrane Glycoproteins with Bisecting Glycosylation from Ovarian Cancer Tissues Reveals Novel Structures and Functions

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• 作者：Heba Allam ; Kazuhiro Aoki ; Benedict B. Benigno ; John F. McDonald ; Samuel G. Mackintosh ; Michael Tiemeyer ; Karen L. Abbott
• 刊名：Journal of Proteome Research
• 出版年：2015
• 出版时间：January 2, 2015
• 年：2015
• 卷：14
• 期：1
• 页码：434-446
• 全文大小：546K
• ISSN：1535-3907

文摘

Biomarkers capable of detecting and targeting epithelial ovarian cancer cells for diagnostics and therapeutics would be extremely valuable. Ovarian cancer is the deadliest reproductive malignancy among women in the U.S., killing over 14鈥?00 women each year. Both the lack of presenting symptoms and high mortality rates illustrate the need for earlier diagnosis and improved treatment of this disease. The glycosyltransferase enzyme GnT-III encoded by the Mgat3 gene is responsible for the addition of GlcNAc (N-acetylglucosamine) to form bisecting N-linked glycan structures. GnT-III mRNA expression is amplified in ovarian cancer tissues compared with normal ovarian tissue. We use a lectin capture strategy coupled to nano-ESI鈥揜PLC鈥揗S/MS to isolate and identify the membrane glycoproteins and unique glycan structures associated with GnT-III amplification in human ovarian cancer tissues. Our data illustrate that the majority of membrane glycoproteins with bisecting glycosylation are common to both serous and endometrioid histological subtypes of ovarian cancer, and several have been reported to participate in signaling pathways such as Notch, Wnt, and TGF尾.