文摘
The biochemical effects of acute and chronic psychological stress have been investigated in maleSprague-Dawley rats using a combination of 1H NMR spectral analysis of plasma and conventionalhematological analyses. Animals were subjected to 35 consecutive days of 6-h sessions of stress, andfollowing a 9 day break, were stressed for a further 6-h period. Plasma samples were collected at 0, 1,3, and 6 h on days 1, 9, 21, 35, and 44, measured using 600 MHz 1H NMR spectroscopy, and analyzedby Principal Components Analysis. Time-dependent biochemical effects of psychological stress on arange of endogenous metabolites were evident and were correlated with the intensity of the stressresponse as defined by corticosterone and hematological parameters. Following acute stress, increasesin the levels of glucose and ketone bodies, and decreases in the levels of acetate, alanine, isoleucine,lactate, leucine, valine, and lipoproteins, were observed. Chronic stress-induced increases in plasmalevels of alanine, lactate (day 9), and leucine, valine, and choline (day 44) and decreases in acetate(day 9) and lipoprotein concentrations were observed. Positive correlations between plasma corticosterone level and glucose and glycerol, and between plasma lipoprotein concentrations and hemoglobinlevels, were established using Projection to Latent Structures (PLS) analysis. This study indicates thepotential of using NMR-based metabonomic strategies for the characterization of endogenous metabolicperturbations induced by psychological stressors and lifestyle choices.
