Transgenic Indian Mustard Overexpressing Selenocysteine Lyase or Selenocysteine Methyltransferase Exhibit Enhanced Potential for Selenium Phytoremediation under Field Conditions

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• 作者：Gary Ba&ntilde ; uelos ; Danika L. LeDuc ; Elizabeth A. H. Pilon-Smits ; Norman Terry
• 刊名：Environmental Science & Technology
• 出版年：2007
• 出版时间：January 15, 2007
• 年：2007
• 卷：41
• 期：2
• 页码：599 - 605
• 全文大小：242K
• 年卷期：v.41,no.2(January 15, 2007)
• ISSN：1520-5851

文摘

Two new transgenic Indian mustard [Brassica juncea (L.)Czern.] lines were tested under field conditions fortheir ability to accumulate selenium (Se) from Se- and boron-contaminated saline sediment. The transgenic linesoverexpress genes encoding the enzymes selenocysteinelyase (cpSL) and selenocysteine methyltransferase(SMT), respectively. In the first Spring planting, cpSL,SMT, and wildtype plants (WT) were compared, while SMTand WT were compared in a second, Fall planting. Inthe Spring planting, shoots of the cpSL transgenic plantsaccumulated 2-fold more Se (p < 0.01), had 1.8 times higherleaf Se concentrations (p < 0.01), and grew better oncontaminated soil than WT. The SMT plants had a 1.7-fold higher leaf Se concentration than WT (p < 0.05). Inthe Fall planting, the SMT transgenic plants accumulated 1.6-fold more Se in their shoots than WT (p < 0.01) with Seconcentrations being higher in both leaves and stems. Theseresults conclusively demonstrate that cpSL and SMTtransgenic lines have significantly greater Se phytoremediationpotential than wildtype Indian mustard. Further, thisstudy confirms the importance of field testing for evaluatingfuture transgenic lines.