A Homogeneous Chemiluminescent Immunoassay Method

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• 作者：Hashem Akhavan-Tafti ; Dean G. Binger ; John J. Blackwood ; Ying Chen ; Richard S. Creager ; Renuka de Silva ; Robert A. Eickholt ; Jose E. Gaibor ; Richard S. Handley ; Kenneth P. Kapsner ; Senja K. Lopac ; Michael E. Mazelis ; Terri L. McLernon ; James D. Mendoza ; Bruce H. Odegaard ; Sarada G. Reddy ; Michael Salvati ; Barry A. Schoenfelner ; Nir Shapir ; Katherine R. Shelly ; Jeff C. Todtleben ; Guoping Wang ; Wenhua Xie
• 刊名：The Journal of the American Chemical Society
• 出版年：2013
• 出版时间：March 20, 2013
• 年：2013
• 卷：135
• 期：11
• 页码：4191-4194
• 全文大小：244K
• 年卷期：v.135,no.11(March 20, 2013)
• ISSN：1520-5126

文摘

A new homogeneous chemiluminescent immunoassay method featuring the use of specific binding members separately labeled with an acridan-based chemiluminescent compound and a peroxidase is reported. Formation of an immunocomplex brings the chemiluminescent compound and the peroxidase into close proximity. Without any separation steps, a chemiluminescent signal is generated upon addition of a trigger solution, and the intensity is directly correlated to the quantity of the analyte.