Film Thickness Dependence of Protein Adsorption from Blood Serum and Plasma onto Poly(sulfobetaine)-Grafted Surfaces

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• 作者：Wei Yang ; Shengfu Chen ; Gang Cheng ; Hana Vaisocherová ; Hong Xue ; Wei Li ; Jinli Zhang ; Shaoyi Jiang
• 刊名：Langmuir
• 出版年：2008
• 出版时间：September 2, 2008
• 年：2008
• 卷：24
• 期：17
• 页码：9211-9214
• 全文大小：118K
• 年卷期：v.24,no.17(September 2, 2008)
• ISSN：1520-5827

文摘

In this work, we investigate protein adsorption from single protein solutions and complex media such as 100% blood serum and plasma onto poly(sulfobetaine methacrylate) (polySBMA)-grafted surfaces via atom transfer radical polymerization (ATRP) at varying film thicknesses. It is interesting to observe that protein adsorption exhibits a minimum at a medium film thickness. Results show that the surface with 62 nm polySBMA brushes presents the best nonfouling character in 100% blood serum and plasma although all of these surfaces are highly resistant to nonspecific protein adsorption from single fibrinogen and lysozyme solutions. Surface resistance to 100% blood serum or plasma is necessary for many applications from blood-contacting devices to drug delivery. This work provides a new in vitro evaluation standard for the application of biomaterials in vivo.