Active Site Architecture of a Sugar N-Oxygenase

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• 作者：James B. Thoden ; Megan C. Branch ; Alex L. Zimmer ; Nathan A. Bruender ; Hazel M. Holden
• 刊名：Biochemistry
• 出版年：2013
• 出版时间：May 14, 2013
• 年：2013
• 卷：52
• 期：19
• 页码：3191-3193
• 全文大小：254K
• 年卷期：v.52,no.19(May 14, 2013)
• ISSN：1520-4995

文摘

KijD3 is a flavin-dependent N-oxygenase implicated in the formation of the nitro-containing sugar d-kijanose, found attached to the antibiotic kijanimicin. For this investigation, the structure of KijD3 in complex with FMN and its dTDP-sugar substrate was solved to 2.1 脜 resolution. In contrast to the apoenzyme structure, the C-terminus of the protein becomes ordered and projects into the active site cleft [Bruender, N. A., Thoden, J. B., and Holden, H. M. (2010) Biochemistry 49, 3517鈥?524]. The amino group of the dTDP-aminosugar that is oxidized is located 4.9 脜 from C4a of the flavin ring. The model provides a molecular basis for understanding the manner in which KijD3 catalyzes its unusual chemical transformation.