DOF AFFECTING GERMINATION 2 is a positive regulator of light-mediated seed germination and is repressed by DOF AFFECTING GERMINATION 1

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• 作者：Silvia Santopolo (1)
  Alessandra Boccaccini (1) (2)
  Riccardo Lorrai (1) (2)
  Veronica Ruta (1)
  Davide Capauto (1)
  Emanuele Minutello (1)
  Giovanna Serino (1)
  Paolo Costantino (1)
  Paola Vittorioso (1) (2)
  
  1. Dipartimento di Biologia e Biotecnologie 鈥淐. Darwin鈥? Sapienza Universit脿 di Roma ; Piazzale Aldo Moro 5 ; 00185 ; Rome ; Italy
  2. Istituto Pasteur Fondazione Cenci Bolognetti ; Dipartimento di Biologia e Biotecnologie 鈥淐. Darwin鈥? Sapienza Universit脿 di Roma ; Piazzale Aldo Moro 5 ; 00185 ; Rome ; Italy
  
• 关键词：DAG2 ; Seed germination ; DAG1 ; Arabidopsis thaliana
• 刊名：BMC Plant Biology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：15
• 期：1
• 全文大小：1,467 KB
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• 刊物主题：Plant Sciences; Agriculture; Tree Biology;
• 出版者：BioMed Central
• ISSN：1471-2229

文摘

Background The transcription factor DOF AFFECTING GERMINATION1 (DAG1) is a repressor of the light-mediated seed germination process. DAG1 acts downstream PHYTOCHROME INTERACTING FACTOR3-LIKE 5 (PIL5), the master repressor, and negatively regulates gibberellin biosynthesis by directly repressing the biosynthetic gene AtGA3ox1. The Dof protein DOF AFFECTING GERMINATION (DAG2) shares a high degree of aminoacidic identity with DAG1. While DAG1 inactivation considerably increases the germination capability of seeds, the dag2 mutant has seeds with a germination potential substantially lower than the wild-type, indicating that these factors may play opposite roles in seed germination. Results We show here that DAG2 expression is positively regulated by environmental factors triggering germination, whereas its expression is repressed by PIL5 and DAG1; by Chromatin Immuno Precipitation (ChIP) analysis we prove that DAG1 directly regulates DAG2. In addition, we show that Red light significantly reduces germination of dag2 mutant seeds. Conclusions In agreement with the seed germination phenotype of the dag2 mutant previously published, the present data prove that DAG2 is a positive regulator of the light-mediated seed germination process, and particularly reveal that this protein plays its main role downstream of PIL5 and DAG1 in the phytochrome B (phyB)-mediated pathway.