Screening the Toxicity and Biodegradability of Petroleum Hydrocarbons by a Rapid Colorimetric Method

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• 作者：Renato Nallin Montagnolli (1)
  Paulo Renato Matos Lopes (1)
  Ederio Dino Bidoia (1)
  
  1. Departamento de Bioqu铆mica e Microbiologia ; Instituto de Bioci锚ncias ; UNESP 鈥?University Estadual Paulista ; Avenida 24 A ; 1515 鈥?Bela Vista ; Rio Claro ; SP ; CEP 13506-900 ; Brazil
  
• 刊名：Archives of Environmental Contamination and Toxicology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：68
• 期：2
• 页码：342-353
• 全文大小：512 KB
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• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Terrestrial Pollution
  Agriculture
  Ecology
  Forestry
  Environment
  Soil Science and Conservation
  
• 出版者：Springer New York
• ISSN：1432-0703

文摘

Crude oil and petroleum products have a wide variety of hazardous components with high toxicity and low biodegradability. Certain dyes change their colors by intercepting electron transfer reactions during the transformation processes. This study applied resazurin and 2,6-dichlorophenol-indophenol indicators for a rapid screening biodegradation capability and toxicity response to various petroleum products such as motor oil, diesel, gasoline, and phenol. Colorimetry tests were performed in test tubes, and the absorbance values were measured over time. We observed different discoloration profiles after degradation tests using Bacillus subtilis inoculum. Phytotoxicity assays were also performed to compare colorimetric screening assays with a conventional toxicity testing with plants (seed germination). The results indicated that biotransformation of oils can increase its overall toxicity. Intermediate byproducts can be formed through biodegradation and thereby increase the toxicity of oils. The assessment of acute toxicity has shown that phenol is extremely toxic to petroleum-biodegrading microbial communities. Low molecular-weight gasoline was considered biodegradable, but it also exhibited a high acute toxic effect, mainly due to its high solubility and the presence of more volatile compounds that can penetrate cells and potentially damage cellular structures.