The yeast Komagataella: A genetic genus in accordance with interspecies hybridization
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  • 作者:G. I. Naumov
  • 关键词:Komagataella yeasts ; Pichia pastoris ; interspecies hybridization ; auxotrophic mutants ; sibling species
  • 刊名:Microbiology
  • 出版年:2015
  • 出版时间:July 2015
  • 年:2015
  • 卷:84
  • 期:4
  • 页码:538-543
  • 全文大小:322 KB
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  • 作者单位:G. I. Naumov (1) (2)

    1. Research and Educational Center for Biomedical Technologies, All-Russian Research Institute of Medicinal and Aromatic Plants, Russian Academy of Sciences, Moscow, Russia
    2. State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia
  • 刊物类别:Biomedical and Life Sciences
  • 刊物主题:Life Sciences
    Microbiology
    Medical Microbiology
    Russian Library of Science
  • 出版者:MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
  • ISSN:1608-3237
文摘
Using induced complementary auxotrophic mutants and selective growth of prototrophic hybrids on minimal medium, hybridization of the type strain of Komagataella kurtzmanii VKPM Y-727 with the type strains of K. pastoris VKPM Y-3262, K. phaffii NRRL Y-7556, K. populi NRRL YB-455, K. pseudopastoris NRRL Y-27603, and K. ulmi NRRL YB-407 was demonstrated. The data obtained suggest that the genus Komagataella, established previously by phylogenetic analysis, corresponds well to the concept of genetic genus in ascomycetous fungi. According to this concept, a genetic genus is a group of hybridized species having a common mating type system. Application of the concept of genetic genus for different yeast genera is discussed.
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