Experimental study on cryotherapy for fungal corneal ulcer

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• 作者：Yingxin Chen (1)
  Weijia Yang (2)
  Minghong Gao (1)
  Michael Wellington Belin (3)
  Hai Yu (1)
  Jing Yu (1)
  
  1. Department of Ophthalmology ; General Hospital of Shenyang Military Area Command ; No. 83 Wenhua Road ; Shenhe District ; Shenyang ; 110840 ; China
  2. Dalian Medical University ; 9 West Lvshun South Road ; Dalian ; 116044 ; China
  3. Department of Ophthalmology ; University of Arizona ; Arizona Health Sciences Center ; 655 N. AlvernonWay ; Suite 108 ; Tucson ; AZ ; 85711 ; USA
  
• 关键词：Fungal corneal keratitis ; Cryotherapy ; Anti ; fungal agents
• 刊名：BMC Ophthalmology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：15
• 期：1
• 全文大小：2,322 KB
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• 刊物主题：Ophthalmology;
• 出版者：BioMed Central
• ISSN：1471-2415

文摘

Background Fungal corneal ulcer is one of the major causes of visual impairment worldwide. Treatment of fungal corneal ulcer mainly depends on anti-fungal agents. In the current study, we developed an integrated combination therapy of cryotherapy and anti-fungal agents to facilitate effective treatment of fungal corneal ulcer. Methods Rabbit models of cornea infection were established using a combined method of intrastromal injection and keratoplasty. After treatment with cryotherapy and anti-fungal agents, scanning electron microscopy, transmission electron microscopy, and confocal microscopy were conducted to observe changes in microstructure in the rabbits. Periodic acid Schiff A and hematoxylin and eosin staining were used for detection of histological changes. Results Continuous scanning electron microscopy and transmission electron microscopy observations showed that cryothermal treatment inhibited growth of fungal mycelium by destroying fungal cellular structures. Typical cryotherapy was effective in curing fungal corneal ulcer. Different fungi showed different susceptibilities to treatment. The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst. Conclusions Our study provides a novel method of a combination of cryotherapy and anti-fungal agents for treatment of fungal corneal ulcer. This treatment could help facilitate the practice of fungal keratitis treatment in the future.