Investigation of malignant transformation of sporadic adenomas of colon by fluorimetry

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• 作者：Yu. S. Korneva ; A. E. Dorosevich ; V. S. Maryakhina
• 关键词：Colon cancer ; Fluorimetry ; Cancerogenesis ; Adenoma ; Adenocarcinoma
• 刊名：Lasers in Medical Science
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：32
• 期：2
• 页码：363-367
• 全文大小：
• 刊物类别：Medicine
• 刊物主题：Medicine/Public Health, general; Dentistry; Optics, Lasers, Photonics, Optical Devices; Quantum Optics;
• 出版者：Springer London
• ISSN：1435-604X
• 卷排序：32

文摘

In this work, biochemical changes in sporadic adenoma during progression to colon cancer have been detected by spectral techniques. It is shown that spectra of all types of adenomas as well as adenocarcinoma have two maxima at 265 ÷ 270 nm and at 330 ÷ 340 nm. The first maximum is primarily defined by tryptophan containing peptides. The second maximum of the spectra is in the main defined by collagen presence in stroma of tumors and normal mucosa. Progression of precancer lesions to advanced cancer leads to increase of NADH concentration impacting on the second maximum of spectra. At the time, the spectra of both AC differ from each other by widths of the second maximum. For well-differentiated AC, the second maximum is wider. It gives reason to suggest that two fluorophores impact on its intensity. These fluorophores can be NADH and vitamin B₆. Correlation between microscopical and spectral results can allow to use fluorimetry for detail diagnostics and investigation of colon cancer development.