Comparative analysis of macrophage migration inhibitory factors (MIFs) from the parasitic nematode Onchocerca volvulus and the free-living nematode Caenorhabditis elegans

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• 作者：Irene Ajonina-Ekoti (1)
  Marc Andre Kurosinski (1)
  Abuelhassan Elshazly Younis (2) (3)
  Dieudonne Ndjonka (4)
  Manchang Kingsley Tanyi (2) (5)
  Mbunkah Achukwi (5)
  Albert Eisenbarth (6)
  Caroline Ajonina (1)
  Kai Lüersen (1)
  Minka Breloer (2)
  Norbert W Brattig (2)
  Eva Liebau (1)
  
• 刊名：Parasitology Research
• 出版年：2013
• 出版时间：September 2013
• 年：2013
• 卷：112
• 期：9
• 页码：3335-3346
• 全文大小：653KB
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• 作者单位：Irene Ajonina-Ekoti (1)
  Marc Andre Kurosinski (1)
  Abuelhassan Elshazly Younis (2) (3)
  Dieudonne Ndjonka (4)
  Manchang Kingsley Tanyi (2) (5)
  Mbunkah Achukwi (5)
  Albert Eisenbarth (6)
  Caroline Ajonina (1)
  Kai Lüersen (1)
  Minka Breloer (2)
  Norbert W Brattig (2)
  Eva Liebau (1)
  
  1. Institute of Animal Physiology, University of Münster, Schlossplatz 8, 48143, Münster, North Rhine-Westphalia, Germany
  2. Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, 20359, Hamburg, Germany
  3. Zoology Department, Faculty of Science, Aswan University, 81582, Aswan, Egypt
  4. Faculty of Science, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon
  5. Institute of Agricultural Research for Development, Wakwa Regional Centre, PO Box 65, Ngaoundere, Cameroon
  6. Institute of Evolution and Ecology, University of Tübingen, Auf der Morgenstelle 28, 74074, Tübingen, Baden-Württemberg, Germany
  

文摘

The macrophage migration inhibitory factors (MIFs) from the filarial parasite Onchocerca volvulus (OvMIF) were compared to the MIFs from the free-living nematode Caenorhabditis elegans (CeMIF) with respect to molecular, biochemical and immunological properties. Except for CeMIF-4, all other MIFs demonstrated tautomerase activity. Surprisingly, OvMIF-1 displayed oxidoreductase activity. The strongest immunostaining for OvMIF-1 was observed in the outer cellular covering of the adult worm body, the syncytial hypodermis; moderate immunostaining was observed in the uterine wall. The generation of a strong humoral immune response towards OvMIF-1 and reduced reactivity to OvMIF-2 was indicated by high IgG levels in patients infected with O. volvulus and cows infected with the closely related Onchocerca ochengi, both MIFs revealing identical amino acid sequences. Using Litomosoides sigmodontis-infected mice, a laboratory model for filarial infection, MIFs derived from the tissue-dwelling O. volvulus, the rodent gut-dwelling Strongyloides ratti and from free-living C. elegans were recognized, suggesting that L. sigmodontis MIF-specific IgM and IgG1 were produced during L. sigmodontis infection of mice and cross-reacted with all MIF proteins tested. Thus, MIF apparently functions as a target of B cell response during nematode infection, but in the natural Onchocerca-specific human and bovine infection, the induced antibodies can discriminate between MIFs derived from parasitic or free-living nematodes.