Molecular characterization and expression analysis of a gene encoding an isoamylase-type starch debranching enzyme 3 (ISA3) in grain amaranths

详细信息    查看全文

• 作者：Young-Jun Park (1)
  Kazuhiro Nemoto (2)
  Norihiko Tomooka (1)
  Tomotaro Nishikawa (1)
  
• 关键词：Amaranth ; Starch debranching enzyme ; Expression
• 刊名：Molecular Breeding
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：33
• 期：4
• 页码：793-802
• 全文大小：874 KB
• 参考文献：1. Ball SG, Morell MK (2003) From bacterial glycogen to starch: understanding the biogenesis of the plant starch granule. Annu Rev Plant Biol 54:207-33 CrossRef
  2. Bierhals J, Lajolo F, Cordenunsi B (2004) Activity, cloning, and expression of an isoamylase-type starch-debranching enzyme from banana fruit. J Agric Food Chem 52:7412-418 CrossRef
  3. Burton RA, Jenner H, Carrangis L, Fahy B (2002) Starch granule initiation and growth are altered in barley mutants that lack isoamylase activity. Plant J 31:97-12 CrossRef
  4. Bustos R (2004) Starch granule initiation is controlled by a heteromultimeric isoamylase in potato tubers. Proc Natl Acad Sci USA 101:2215-220 CrossRef
  5. Chan K, Sun M (1997) Genetic diversity and relationships detected by isozyme and RAPD analysis of crop and wild species of / Amaranthus. Theor Appl Genet 95:865-73 CrossRef
  6. Costea M, Brenner D, Tardif F, Tan Y, Sun M (2006) Delimitation of / Amaranthus cruentus L. and / Amaranthus caudatus L. using micromorphology and AFLP analysis: an application in germplasm identification. Genet Resour Crop Evol 53:1625-633 CrossRef
  7. Dauvillée D, Colleoni C, Mouille G, Morell MK, d’Hulst C, Wattebled F, Liénard L, Delvallé D, Ral J-P, Myers AM, Ball SG (2001) Biochemical characterization of wild-type and mutant isoamylases of / Chlamydomonas reinhardtii supports a function of the multimeric enzyme organization in amylopectin maturation. Plant Physiol 125:1723-731 CrossRef
  8. Delatte T, Trevisan M, Parker ML, Zeeman SC (2005) Arabidopsis mutants / Atisa1 and / Atisa2 have identical phenotypes and lack the same multimeric isoamylase, which influences the branch point distribution of amylopectin during starch synthesis. Plant J 41:815-30 CrossRef
  9. Hussain H, Mant A, Seale R, Zeeman S, Hinchliffe E, Edwards A, Hylton C, Bornemann S, Smith AM, Martin C, Bustos R (2003) Three isoforms of isoamylase contribute different catalytic properties for the debranching of potato glucans. Plant Cell 15:133-49 CrossRef
  10. James MG, Robertson DS, Myers AM (1995) Characterization of the maize gene / sugary1, a determinant of starch composition in kernels. Plant Cell 7:417-29
  11. Joshi C (1987) Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis. Nucl Acids Res 15:9627-640
  12. Kubo A, Colleoni C, Dinges JR, Lin Q, Lappe RR, Rivenbark JG, Meyer AJ, Ball SG, James MG, Hennen-Bierwagen TA, Myers AM (2010) Functions of heteromeric and homomeric isoamylase-type starch-debranching enzymes in developing maize endosperm. Plant Physiol 153:956-69 CrossRef
  13. Lorenz K (1981) / Amarantus hypochondriacus—characteristics of the starch and baking potential of the flour. Starch-St?rke 33:149-53 CrossRef
  14. Marcone MF (2001) Starch properties of / Amaranthus pumilus (seabeach amaranth): a threatened plant species with potential benefits for the breeding/amelioration of present / Amaranthus cultivars. Food Chem 73:61-6 CrossRef
  15. Mouille G, Maddelein ML, Libessart N, Talaga P, Decq A, Delrue B, Ball S (1996) Preamylopectin processing: a mandatory step for starch biosynthesis in plants. Plant Cell 8:1353-366
  16. Myers AM, Morell MK, James MG, Ball SG (2000) Recent progress toward understanding biosynthesis of the amylopectin crystal. Plant Physiol 122(4):989-98 CrossRef
  17. Nakamura Y, Kubo A, Shimamune T, Matsuda T, Harada K, Satoh H (1997) Correlation between activities of starch debranching enzyme and alpha-polyglucan structure in endosperms of / sugary- / 1 mutants of rice. Plant J 12:143-53 CrossRef
  18. Ohdan T, Francisco PB, Sawada T, Hirose T, Terao T, Satoh H, Nakamura Y (2005) Expression profiling of genes involved in starch synthesis in sink and source organs of rice. J Exp Bot 56:3229-244 CrossRef
  19. Park Y-J, Nishikawa T (2012) Characterization and expression analysis of the starch synthase gene family in grain amaranth ( / Amaranthus cruentus L.). Genes Genet Syst 87:281-89 CrossRef
  20. Park Y-J, Nemoto K, Nishikawa T, Matsushima K, Minami M, Kawase M (2009) Molecular cloning and characterization of granule bound starch synthase I cDNA from a grain amaranth ( / Amaranthus cruentus L.). Breed Sci 59:351-60 CrossRef
  21. Park Y-J, Nemoto K, Nishikawa T, Matsushima K, Minami M, Kawase M (2010) Waxy strains of three amaranth grains raised by different mutations in the coding region. Mol Breed 25:623-35 CrossRef
  22. Park Y-J, Nemoto K, Nishikawa T, Matsushima K, Minami M, Kawase M (2011) Genetic diversity and expression analysis of granule bound starch synthase I gene in the new world grain amaranth ( / Amaranthus cruentus L.). J Cereal Sci 53:298-05 CrossRef
  23. Park Y-J, Nishikawa T, Tomooka N, Nemoto K (2012) Molecular cloning and expression analysis of a gene encoding soluble starch synthase I from grain amaranth ( / Amaranthus cruentus L.). Mol Breed 30:1065-076 CrossRef
  24. Posewitz MC, Smolinski SL, Kanakagiri S, Melis A, Seibert M, Ghirardi ML (2004) Hydrogen photoproduction is attenuated by disruption of an isoamylase gene in / Chlamydomonas reinhardtii. Plant Cell 16:2151-163 CrossRef
  25. Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365-86
  26. Saunders RM, Becker R (1984) Amaranthus: a potential food and feed resource. Adv Cereal Sci Technol 6:357-96
  27. Smith A, Denyer K, Martin C (1997) The synthesis of the starch granule. Annu Rev Plant Biol 48:67-7 CrossRef
  28. Stone LA, Lorenz K (1984) The starch of amaranthus—physico-chemical properties and functional characteristics. Starch-St?rke 36:232-37 CrossRef
  29. Streb S, Delatte T, Umhang M, Eicke S, Schorderet M, Reinhardt D, Zeeman SC (2008) Starch granule biosynthesis in Arabidopsis is abolished by removal of all debranching enzymes but restored by the subsequent removal of an endoamylase. Plant Cell 20:3448-466 CrossRef
  30. Swofford D (1988) PAUP*: phylogenetic analysis using parsimony and other methods, version 4.0 (test ver. 61-4). Sinauer Associates Publishers, Sunderland
  31. Thompson J, Gibson T, Plewniak F, Jeanmougin F, Higgins D (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 25:4876
  32. van der Maarel MJEC, van der Veen B, Uitdehaag JCM, Leemhuis H, Dijkhuizen L (2002) Properties and applications of starch-converting enzymes of the alpha-amylase family. J Biotechnol 94:137-55 CrossRef
  33. Wattebled F, Dong Y, Dumez S, Delvallé D, Planchot V, Berbezy P, Vyas D, Colonna P, Chatterjee M, Ball S, d’Hulst C (2005) Mutants of arabidopsis lacking a chloroplastic isoamylase accumulate phytoglycogen and an abnormal form of amylopectin. Plant Physiol 138:184-95 CrossRef
  34. Xu F, Sun M (2001) Comparative analysis of phylogenetic relationships of grain amaranths and their wild relatives ( / Amaranthus; Amaranthaceae) using internal transcribed spacer, amplified fragment length polymorphism, and double-primer fluorescent intersimple sequence repeat markers. Mol Phylogenet Evol 21:372-87 CrossRef
  35. Yun MS, Umemoto T, Kawagoe Y (2011) Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis. Plant Cell Physiol 52(6):1068-082 CrossRef
  36. Zheleznov AV, Solonenko LP, Zheleznova NB (1997) Seed proteins of the wild and the cultivated Amaranthus species. Euphytica 97(2):177-82 CrossRef
  
• 作者单位：Young-Jun Park (1)
  Kazuhiro Nemoto (2)
  Norihiko Tomooka (1)
  Tomotaro Nishikawa (1)
  
  1. Genetic Resources Center, National Institute of Agrobiological Sciences, 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan
  2. Graduate School of Agriculture, Shinshu University, 8304, Minamiminowa, Nagano, 399-4598, Japan
  
• ISSN：1572-9788

文摘

A cDNA clone from amaranth perisperm that encodes an isoamylase (ISA)-type starch debranching enzyme 3 was isolated and analyzed for the first time. The cDNA consisted of 2,715?bp with a single open reading frame of 2,346?bp, encoding a protein of 781 amino acid residues. The deduced amino acid sequence of CrISA3 shared 63-1?% identity with those of other plant ISA3s. We also investigated the genetic diversity of ISA3 in three species of grain amaranth. A comparison of their ISA3 coding sequences revealed an extremely high level of conservation and only 11 single nucleotide polymorphisms were detected. The expression of the CrISA3 gene in amaranth developmental seeds and several tissues was investigated by qRT-PCR analysis. The results showed that CrISA3 was rapidly expressed at the early stage during seed maturation. It was also expressed in non-storage tissues (leaf, petiole, stem, and root) as well as in storage tissue. This observation demonstrates that CrISA3 may play an important role in perisperm starch accumulation at the early developmental stages. In addition, our results indicate that CrISA3 plays important roles in the synthesis of storage and transitory starches. The characterization of the CrISA3 gene will contribute to further studies on starch biosynthesis in Amaranthus.