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Optimized Microwave Assisted Synthesis of LL37, a Cathelicidin Human Antimicrobial Peptide
- 作者：Jyotibon Dutta ; Suhas Ramesh…
- 关键词：LL37 ; SPPS ; Microwave ; DIC ; OxymaPure ; THF
- 刊名：International Journal of Peptide Research and Therapeutics
- 出版年：2015
- 出版时间：March 2015
- 年：2015
- 卷：21
- 期：1
- 页码：13-20
- 全文大小：634 KB
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16. Johansson J, Gudmundsson GH, Rottenberg ME, Berndt KD, Agerberth B (1998) Conformation-dependent antibacterial activity of the naturally occurring human peptide LL-37. J Biol Chem 273:3718-724 CrossRef
17. Kajiya M, Shiba H, Komatsuzawa H, Ouhara K, Fujita T, Takeda K, Uchida Y, Mizuno N, Kawaguchi H, Kurihara H (2010) The antimicrobial peptide LL37 induces the migration of human pulp cells: a possible adjunct for regenerative endodontics. J Endodont 36:1009-013 Antimicrobial Research Unit, School of Health Sciences, University of KwaZulu-Natal, Durban, 4001, South Africa
4. School of Chemistry, Yachay Tech, Yachay City of Knowledge, 100119, Urcuqui, Ecuador
3. Institute for Research in Biomedicine and CIBER-BBN, 08028, Barcelona, Spain
5. School of Chemistry and Physics, University of KwaZulu-Natal, Durban, 4001, South Africa
6. Department of Organic Chemistry, University of Barcelona, 08028, Barcelona, Spain
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Biochemistry
Animal Anatomy, Morphology and Histology
Polymer Sciences
- 出版者：Springer Netherlands
- ISSN：1573-3904
文摘
LL37, a human cathelecidin antimicrobial peptide comprising of?37 amino acids has emerged as one of the vital therapeutic peptides associated with a number of biological applications. In this context, we herein report a highly efficient and optimized methodology for the synthesis of LL37 through SPPS assisted by microwave power. Standard conditions employing uronium coupling reagents was unsatisfactory from the 20th amino acid residue onwards. A segmentation approach revealed that the amide bond formation between the Val and Ile was identified as the problematic coupling. It was found that DIC/OxymaPure in conjunction with THF as the solvent gave best results during manual coupling of 20th position Ile and required double coupling as revealed by HPLC and MALDI-TOF MS. In order to verify the synthesis, antibacterial testing was carried out and the results revealed comparable values with that of literature reported.
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