A high-throughput expression screening platform to optimize the production of antimicrobial peptides

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• 作者：Christine Schreiber ; Hagen Müller ; Oliver Birrenbach…
• 关键词：Antimicrobial peptides ; Expression screening ; Recombinant protein production ; Golden Gate cloning ; Combinatorial library ; E. coli ; P. pastoris ; Expression strain ; Promoter ; Fusion protein
• 刊名：Microbial Cell Factories
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：16
• 期：1
• 全文大小：2148KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Applied Microbiology; Biotechnology; Microbiology; Microbial Genetics and Genomics; Enzymology; Genetic Engineering;
• 出版者：BioMed Central
• ISSN：1475-2859
• 卷排序：16

文摘

BackgroundAntimicrobial peptides (AMPs) are promising candidates for the development of novel antibiotics, but it is difficult to produce sufficient quantities for preclinical and clinical studies due to their toxicity towards microbial expression hosts. To avoid laborious trial-and-error testing for the identification of suitable expression constructs, we have developed a small-scale expression screening platform based on a combinatorial plasmid library.