Quantitative Characterization of Bovine Serum Albumin, ¦Á-Lactalbumin and ¦Â-Lactoglobulin in Commercial Whey Sample by RP-LC

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• 作者：Goutam Mukhopadhyay ; Jasmina Khanam ; Arunabha Nanda and Arindam Basu Sarkar
• 关键词：Column liquid chromatography ; Commercial waste whey ; Bovine serum albumin ; α ; Lactalbumin ; β ; lactoglobulin
• 刊名：Chromatographia
• 出版年：2010
• 出版时间：April, 2010
• 年：2010
• 卷：71
• 期：7-8
• 页码：699-702
• 全文大小：280.5 KB

文摘

A simple, sensitive and precise reverse phase liquid chromatographic method has been developed and validated for quantification of bovine serum albumin (BSA), α-lactalbumin (α-La) and β–lactoglobulin (β-Lg) that are removed from whey waste by foam fractionation method. The data is reproducible over a wide concentration range. This optimized method allowed analysis of BSA, α-La, β-Lg in a mixture within 5 min and could be applied to the analysis of a variety of commercial and laboratory whey products within a short time.