Membrane protein reconstitution for functional and structural studies

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• 作者：LiGuo Wang (1)
  LiGe Tonggu (1)
  
  1. Department of Biological Structure ; University of Washington ; Seattle ; Washington ; 98195 ; USA
  
• 关键词：membrane protein ; reconstitution ; Ca2+ signaling ; Ca2+ mediators ; Ca2+ ; regulated enzymes ; Ca2+ transducers
• 刊名：Science China Life Sciences
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：58
• 期：1
• 页码：66-74
• 全文大小：1,684 KB
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• 刊物主题：Life Sciences, general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-1889

文摘

Membrane proteins are involved in various critical biological processes, and studying membrane proteins represents a major challenge in protein biochemistry. As shown by both structural and functional studies, the membrane environment plays an essential role for membrane proteins. In vitro studies are reliant on the successful reconstitution of membrane proteins. This review describes the interaction between detergents and lipids that aids the understanding of the reconstitution processes. Then the techniques of detergent removal and a few useful techniques to refine the formed proteoliposomes are reviewed. Finally the applications of reconstitution techniques to study membrane proteins involved in Ca2+ signaling are summarized.