Alterations of GPI transamidase subunits in head and neck squamous carcinoma

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• 作者：Wei-Wen Jiang (1) (3)
  Marianna Zahurak (2)
  Zeng-Tong Zhou (3)
  Hannah Lui Park (1)
  Zhong-Min Guo (1)
  Guo-Jun Wu (1)
  David Sidransky (1)
  Barry Trink (1)
  Joseph A Califano (1)
  
• 刊名：Molecular Cancer
• 出版年：2007
• 出版时间：December 2007
• 年：2007
• 卷：6
• 期：1
• 全文大小：226KB
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• 作者单位：Wei-Wen Jiang (1) (3)
  Marianna Zahurak (2)
  Zeng-Tong Zhou (3)
  Hannah Lui Park (1)
  Zhong-Min Guo (1)
  Guo-Jun Wu (1)
  David Sidransky (1)
  Barry Trink (1)
  Joseph A Califano (1)
  
  1. Department of Otolaryngology- Head and Neck Surgery, Head and Neck Cancer Research Division, Johns Hopkins Medical Institutions, 601 N. Caroline Street, 6th Floor, Baltimore, MD, 21287-0910, USA
  3. Department of Oral Medicine, The Ninth People's Hospital, School of Stomatology, Shanghai Jiao Tong University, 639 Zhizaoju Road, Huangpu District, Shanghai, Postcode, 200011, China
  2. Department of Oncology Biostatistics, Johns Hopkins Medical Institutions, Suite 1103, 550 North Broadway, Baltimore, 21205-2013, USA
  
• ISSN：1476-4598

文摘

Background GPI anchor attachment is catalyzed by the GPI transamidase (GPIT) complex. GAA1, PIG-T and PIG-U are the three of five GPIT subunits. Previous studies demonstrated amplification and overexpression of GPIT subunits in bladder and breast cancer with oncogenic function. We performed an analysis of these subunits in head and neck squamous cell carcinoma (HNSCC). Results To evaluate GAA1, PIG-T and PIG-U in HNSCC, we used quantitative PCR (QPCR) and quantitative RT-PCR (QRT-PCR) to determine the copy number of those genes in primary tumors and the matching lymphocytes in 28 patients with HNSCC and quantified RNA expression of those genes in 16 primary HNSCC patients and 4 normal control tissue samples. GAA1 showed a significant increase in normalized mRNA expression, 2.11 (95% CI: 1.43, 2.79), in comparison to that of normal controls, 0.43 (95% CI: -0.76, 1.61), p = 0.014 (Mann-Whitney test). The mean genomic copy number of GAA1 was significantly increased in HNSCC, 0.59 (95% CI: 0.50, 0.79), in comparison to lymphocyte DNA, 0.35 (95% CI: 0.30, 0.50), p = 0.001 (paired t-test). Conclusion An increased expression level and elevated copy number for GAA1 suggest a role for this GPI anchor subunit in HNSCC.