Pretreatment with Evans blue, a stimulator of BKCa channels, inhibits compound 48/80-induced shock, systemic inflammation, and mast cell degranulation in the rat

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• 作者：Yaw-Syan Fu ; Su-Yu Kuo ; Hsuan-Yea Lin ; Chun-Lin Chen…
• 关键词：Compound 48/80 ; Allergic shock ; Inflammation ; Evans blue ; Mast cells ; Inhibition
• 刊名：Histochemistry and Cell Biology
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：144
• 期：3
• 页码：237-247
• 全文大小：2,411 KB
• 参考文献：Baluk P, Hirata A, Thurston G, Fujiwara T, Neal CR, Michel CC, McDonald DM (1997) Endothelial gaps: time course of formation and closure in inflamed venules of rats. Am J Physiol 272 (Lung Cell Mol Physiol 16): L155–L170
  Bernik TR, Friedman SG, Ochani M, DiRaimo R, Ulloa L, Yang H, Sudan S, Czura CJ, Ivanova SM, Tracey KJ (2002) Pharmacological stimulation of the cholinergic anti-inflammatory pathway. J Exp Med 195:781-88PubMed Central View Article PubMed
  Bolser DC, Del Prado M, O’Reilly S, Mingo G, Hey JA (1995) Evan’s blue dye blocks capsaicin-induced cough and bronchospasm in the guinea pig. Eur J Pharmacol 276:R1–R3View Article PubMed
  Borovikova LV, Ivanova S, Zhang M, Yang H, Botchkina GI, Watkins LR, Wang H, Abumrad N, Eaton JW, Tracey KJ (2000) Vagus nerve stimulation attenuates the systemic inflammatory response to endotoxin. Nature 405:458-62View Article PubMed
  Brokaw JJ, Hillenbrand CM, White GW, McDonald DM (1990) Mechanism of tachyphylaxis associated with neurogenic plasma extravasation in rat trachea. Am Rev Respir Dis 141:1434-440View Article PubMed
  Chatterjea D, Wetzel A, Mack M, Engblom C, Allen J, Mora-Solano C, Paredes L, Balsells E, Martinov T (2012) Mast cell degranulation mediates compound 48/80-induced hyperalgesia in mice. Biochem Biophys Res Comm 425:237-43PubMed Central View Article PubMed
  Chen IJ, Chiu HF, Huang HT, Teng CM (1984) Edema formation and degranulation of mast cells by Trimeresurus mucrosquamatus snake venom. Toxicon 22:17-8View Article PubMed
  Fu YS, Wang PH, Liu SP, Huang WH, Huang H (2010) Warm SPA-induced hyperthermia confers protection to rats against airway inflammation evoked by capsaicin and substance P. Auton Neurosci 155:49-8View Article PubMed
  Guo JJ, Wang DS, Huang HT (2003) Spontaneous remission of edema and regranulation of goblet cells in rat tracheae after capsaicin-induced acute inflammation. Anat Embryol 206:301-09PubMed
  Hollywood MA, Cotton KD, McHale NG, Thornbury KD (1998) Enhancement of Ca2+-dependent outward current in sheep bladder myocytes by Evans blue dye. Pflugers Arch 435:631-36View Article PubMed
  Huang HT, Guo JJ, Huang YH, Fu YS (2013) Histamine-induced changes in rat tracheal goblet cell mucin store and mucosal edema. Histochem Cell Biol 139:717-26View Article PubMed
  Lidegran M, Domeij S, Carlsoo B, Dahlqvist A (1998) Edema formation in the rat larynx. Cell Tissue Res 292:367-75View Article PubMed
  Liu SP, Chang CY, Huang WH, Fu YS, Chao D, Huang HT (2010) Dimethylthiourea pretreatment inhibits endotoxin-induced compound exocytosis in goblet cells and plasma leakage of rat small intestine. J Electron Micro 59:127-39View Article
  Lo YK, Huang HT (1997) A novel procedure for mediastinal vagotomy inhibits neurogenic inflammation in rat bronchial tree. J Auton Nerv Syst 67:79-8View Article PubMed
  Lundberg JM, Saria A (1983) Capsaicin-induced desensitization of airway mucosa to cigarette smoke, mechanical and chemical irritants. Nature 302:251-53View Article PubMed
  McDonald DM (1994) Endothelial gaps and permeability of venules in rat trachea exposed to inflammatory stimuli. Am J Physiol 266:L61–L83PubMed
  Rohlich P, Anderson P, Uvnas B (1971) Electron microscope observations on compound 48/80-induced degranulation in rat mast cells. Evidence for sequential exocytosis of storage granules. J Cell Biol 51:465-83PubMed Central View Article PubMed
  Saria A, Lundberg JM (1983) Evans blue fluorescence: quantitative and morphological evaluation of vascular permeability in animal tissues. J Neurosci Methods 8:41-9View Article PubMed
  Tam EK, Calonico LD, Nadel JA, McDonald DM (1988) Globule leukocytes and mast cells in the rat trachea: their number, distribution, and response to compound 48/80 and dexamethasone. Anat Embryol 178:107-18View Article PubMed
  Wu SN, Jan CR, Li HF, Chen SA (1999) Stimulation of large-conductance Ca2+-activated K+ channels by Evans blue in cultured endothelial cells of human umbilical veins. Biochem Biophys Res Comm 254:666-74View Article PubMed
  Wu SN, Chen CC, Li HF, Lo YK, Chen SA, Chiang HT (2002) Stimulation of the BKca channel in cultured smooth muscle cells of human trachea by magnolol. Thorax 57:67-4PubMed Central View Article PubMed
  
• 作者单位：Yaw-Syan Fu (1)
  Su-Yu Kuo (2)
  Hsuan-Yea Lin (1)
  Chun-Lin Chen (3)
  Shi-Ying Huang (4)
  Zhi-Hong Wen (4)
  Kun-Zer Lee (3)
  Hung-Tu Huang (2)
  
  1. Department of Biomedical Science and Environmental Biology, College of Life Sciences, Kaohsiung Medical University, Kaohsiung City, Taiwan
  2. Department of Anatomy, School of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan
  3. Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung City, Taiwan
  4. Marine Biomedical Laboratory and Center for Translational Biopharmaceuticals, Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung City, Taiwan
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Anatomy
  Medicine/Public Health, general
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-119X

文摘

The present study demonstrated that intravenous injection of a high dose of compound 48/80 to the rat induced 50?% drop, within a few min, in the mean arterial pressure and pulse pressure as well as systemic inflammatory plasma leakage that might lead to circulatory and respiratory failure. We also investigated whether pretreatment with Evans blue, a stimulator of BKCa channels, could exert inhibitory effect against compound C48/80-induced allergic circulatory shock and systemic inflammation. Different groups of Sprague–Dawley rats received an intravenous injection of a dose of Evans blue (0, 5, 10, or 50?mg/kg) just 20?s prior to injection of compound 48/80 (200?μg/kg, over 2?min). The present study found that pretreatment with Evans blue in a dose of 10 or 50?mg/kg exerted acute inhibitory effect on compound 48/80-induced sudden drop in mean arterial and pulse pressures. We also showed that pretreatment with Evans blue in a dose of 5, 10, or 50?mg/kg significantly inhibited compound 48/80-induced extensive plasma extravasation, mast cell degranulation, and edema formation in various organs including the airways, esophagus, and skin. Pretreatment with Evans blue 50?mg/kg 1?h earlier exhibited longer-term inhibitory effect on compound 48/80-induced arterial hypotension and systemic inflammation. We concluded that Evans blue pretreatment prevented rats from compound 48/80-triggered allergic shock and systemic inflammation, possibly mainly through inhibition of mast cell degranulation. Evans blue might be potentially useful in elucidating the mechanism and acting as a therapeutic agent of allergic shock and systemic inflammation.