Metagenomic analysis of the gut microbiota of the Timber Rattlesnake, Crotalus horridus

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• 作者：Richard William McLaughlin ; Philip A. Cochran ; Scot E. Dowd
• 关键词：Timber Rattlesnake ; Crotalus horridus ; Bacterial diversity ; Metagenomes ; Reptile
• 刊名：Molecular Biology Reports
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：42
• 期：7
• 页码：1187-1195
• 全文大小：797 KB
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• 作者单位：Richard William McLaughlin (1) (2)
  Philip A. Cochran (1)
  Scot E. Dowd (3)
  
  1. Biology Department, Saint Mary’s University of Minnesota, Winona, MN, 55987-1399, USA
  2. General Studies, Gateway Technical College, Kenosha, WI, 53144, USA
  3. Molecular Research LP (MR DNA), Shallowater, TX, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4978

文摘

Snakes are capable of surviving long periods without food. In this study we characterized the microbiota of a Timber Rattlesnake (Crotalus horridus), devoid of digesta, living in the wild. Pyrosequencing-based metagenomics were used to analyze phylogenetic and metabolic profiles with the aid of the MG-RAST server. Pyrosequencing of samples taken from the stomach, small intestine and colon yielded 691696, 957756 and 700419 high quality sequence reads. Taxonomic analysis of metagenomic reads indicated Eukarya was the most predominant domain, followed by bacteria and then viruses, for all three tissues. The most predominant phylum in the domain Bacteria was Proteobacteria for the tissues examined. Functional classifications by the subsystem database showed cluster-based subsystems were most predominant (10-5?%). Almost equally predominant (10-3?%) was carbohydrate metabolism. To identify bacteria in the colon at a finer taxonomic resolution, a 16S rRNA gene clone library was created. Proteobacteria was again found to be the most predominant phylum. The present study provides a baseline for understanding the microbial ecology of snakes living in the wild.