CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production

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• 作者：Brady F. Cress ; Quentin D. Leitz ; Daniel C. Kim…
• 关键词：CRISPRi ; dCas9 ; Transcriptional repression ; Deregulation ; MetJ ; Peonidin 3 ; O ; glucoside ; Anthocyanin O ; methyltransferase ; S ; Adenosyl methionine ; SAM ; AdoMet
• 刊名：Microbial Cell Factories
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：16
• 期：1
• 全文大小：1401KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Applied Microbiology; Biotechnology; Microbiology; Microbial Genetics and Genomics; Enzymology; Genetic Engineering;
• 出版者：BioMed Central
• ISSN：1475-2859
• 卷排序：16

文摘

BackgroundAnthocyanins are a class of brightly colored, glycosylated flavonoid pigments that imbue their flower and fruit host tissues with hues of predominantly red, orange, purple, and blue. Although all anthocyanins exhibit pH-responsive photochemical changes, distinct structural decorations on the core anthocyanin skeleton also cause dramatic color shifts, in addition to improved stabilities and unique pharmacological properties. In this work, we report for the first time the extension of the reconstituted plant anthocyanin pathway from (+)-catechin to O-methylated anthocyanins in a microbial production system, an effort which requires simultaneous co-option of the endogenous metabolites UDP-glucose and S-adenosyl-l-methionine (SAM or AdoMet).