Determination of Aflatoxins M1, M2, B1, B2, G1, and G2 and Ochratoxin A in UHT and Powdered Milk by Modified QuEChERS Method and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry

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• 作者：André Victor Sartori ; Juliana Swensson de Mattos…
• 关键词：Aflatoxins ; Ochratoxin A ; UHT milk ; Powdered milk ; UHPLC ; MS/MS
• 刊名：Food Analytical Methods
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：8
• 期：9
• 页码：2321-2330
• 全文大小：500 KB
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• 作者单位：André Victor Sartori (1)
  Juliana Swensson de Mattos (1)
  Maria Heloísa Paulino de Moraes (1)
  Armi Wanderley da Nóbrega (1)
  
  1. Department of Chemistry, National Institute for Quality Control in Health/Oswaldo Cruz Foundation (INCQS/FIOCRUZ), Rio de Janeiro, RJ, Brazil
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X

文摘

A suitable method for routine analysis of aflatoxins M1, M2, B1, B2, G1, and G2 and ochratoxin A in milk by modified Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) sample treatment method and ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was developed and validated. Satisfactory recoveries ranging from 72 to 121 %, with relative standard deviation lower than 17 % were obtained for the target compounds. The limits of quantification for aflatoxin M1 were 0.017 and 0.25 μg kg? for ultra-high-temperature (UHT) milk and powdered milk, respectively. The validated method was successfully applied to the determination of mycotoxins in milk samples from local supermarkets in Rio de Janeiro, Brazil. Ochratoxin A and aflatoxins B1, B2, G1, and G2 were not detected in the analyzed samples. Aflatoxin M1 was found in 53 (74 %) of the powdered milk samples analyzed at concentration levels ranging from 0.08 to 1.19 μg kg?. In UHT milk, aflatoxin M1 was found in 11 (69 %) of the analyzed samples at concentration levels ranging from 0.005 to 0.042 μg kg?. The concentrations found for aflatoxin M1 in milk samples were lower than the maximum permitted level according to Brazilian regulation. Aflatoxin M2 was detected in 17 (24 %) of the powdered milk samples (>0.08 μg kg?) and in 3 (18 %) of the UHT milk samples (>0.009 μg kg?). The present study is the first report showing the incidence of aflatoxin M2 in milk in Brazil. Keywords Aflatoxins Ochratoxin A UHT milk Powdered milk UHPLC-MS/MS