MicroRNA-484 is more highly expressed in serum of early breast cancer patients compared to healthy volunteers

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• 作者：Silvia Zearo (16)
  Edward Kim (16)
  Ying Zhu (17)
  Jing Ting Zhao (16)
  Stan B Sidhu (16) (18)
  Bruce G Robinson (16) (19)
  Patsy SH Soon (20) (21) (22)
  
  16. Cancer Genetics ; Hormones and Cancer ; Kolling Institute of Medical Research ; University of Sydney ; Sydney ; Australia
  17. Hunter New England Local Health District ; Royal North Shore Hospital ; Sydney ; Australia
  18. Department of Endocrine and Oncology Surgery ; Royal North Shore Hospital ; Sydney ; Australia
  19. Department of Endocrinology ; Royal North Shore Hospital ; Sydney ; Australia
  20. Department of Surgery ; Bankstown Hospital and South Western Sydney Clinical School ; University of New South Wales ; Kensington ; Australia
  21. Bankstown Hospital ; Eldridge Rd ; Bankstown ; NSW ; 2200 ; Australia
  22. Ingham Institute for Applied Medical Research ; Liverpool ; Australia
  
• 关键词：Breast cancer ; microRNA ; Serum
• 刊名：BMC Cancer
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：179 KB
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  29. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/14/200/prepub
  
• 刊物主题：Cancer Research; Oncology; Stem Cells; Animal Models; Internal Medicine;
• 出版者：BioMed Central
• ISSN：1471-2407

文摘

Background Previous studies have profiled breast cancer compared to normal breast tissue and identified differentially expressed microRNAs (miRNAs). These miRNAs are then assessed in serum of breast cancer patients compared to healthy volunteers. MiRNAs in serum however do not always reflect what is in tissue and important serum miRNAs may be missed. PCR arrays were therefore performed on serum samples from breast cancer patients compared to healthy volunteers with the aim of identifying circulating miRNAs that are more highly expressed in serum from early breast cancer patients compared to controls. Methods Taqman low density array (TLDA) cards were used to profile serum miRNAs in a discovery cohort of serum from 39 early breast cancer patients compared to 10 healthy volunteers. The results were confirmed in a validation cohort of serum from 98 early breast cancer patients compared to 25 healthy volunteers using customized qPCR plates. Results Seventeen miRNAs were found to have significantly higher levels in breast cancer serum compared to serum of healthy volunteers in the discovery cohort. Fourteen of these miRNAs were studied in the validation cohort and serum miR-484 was found to be at a significantly higher level in breast cancer serum compared to healthy volunteers. Conclusion In this study, we found that miR-484 is significantly differentially expressed in serum of early breast cancer patients compared to healthy volunteers. We did not however find any correlation between miR-484 levels with histopathological parameters of the breast cancers. With further studies, miR-484 may prove useful as an adjunct to mammography for detection of early breast cancer.