Role of the gerA operon in L-alanine germination of Bacillus licheniformis spores
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  • 作者：Irene S L?vdal (1) (2)
    Cecilie From (2)
    Elisabeth H Madslien (2) (3)
    Kristin Cecilia S Romundset (2)
    Elin Klufterud (2)
    Jan Thomas Rosnes (1)
    Per Einar Granum (2)
  • 刊名：BMC Microbiology
  • 出版年：2012
  • 出版时间：December 2012
  • 年：2012
  • 卷：12
  • 期：1
  • 全文大小：447KB
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  • 作者单位：Irene S L?vdal (1) (2)
    Cecilie From (2)
    Elisabeth H Madslien (2) (3)
    Kristin Cecilia S Romundset (2)
    Elin Klufterud (2)
    Jan Thomas Rosnes (1)
    Per Einar Granum (2)

    1. Nofima AS, Department of Process Technology, M?ltidets hus, Richard Johnsens gate 4, P. Box 8034, N-4068, Stavanger, Norway
    2. Departement of Food Safety and Infection Biology, Section for Food Safety, Norwegian School of Veterinary Science, Ullev?lsveien 72, P. Box 8146 Dep., N-0033, Oslo, Norway
    3. Forsvarets Forskningsinstiutt FFI, Norwegian Defence Research Establishment, P. O. Box 25, N-2027, Kjeller, Norway
  • ISSN：1471-2180
文摘
Background The genome of Bacillus licheniformis DSM 13 harbours three neighbouring open reading frames showing protein sequence similarities to the proteins encoded from the Bacillus subtilis subsp. subtilis 168 gerA operon, GerAA, GerAB and GerAC. In B. subtilis, these proteins are assumed to form a germinant receptor involved in spore germination induced by the amino acid L-alanine. Results In this study we show that disruption of the gerAA gene in B. licheniformis MW3 hamper L-alanine and casein hydrolysate-triggered spore germination, measured by absorbance at 600 nm and confirmed by phase contrast microscopy. This ability was restored by complementation with a plasmid-borne copy of the gerA locus. Addition of D-alanine in the casein hydrolysate germination assay abolished germination of both B. licheniformis MW3 and the complementation mutant. Germination of both B. licheniformis MW3 and the gerA disruption mutant was induced by the non-nutrient germinant Ca2+-Dipicolinic acid. Conclusions These results demonstrate that the B. licheniformis MW3 gerA locus is involved in germination induced by L-alanine and potentially other components present in casein hydrolysate.
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