Fate of cerium dioxide nanoparticles in endothelial cells: exocytosis

详细信息    查看全文

• 作者：Claudia Strobel ; Hartmut Oehring ; Rudolf Herrmann…
• 关键词：Cerium dioxide ; Endothelial cells ; Exocytosis ; Exocytosis inhibitor ; Nanoparticle ; Health effects
• 刊名：Journal of Nanoparticle Research
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：17
• 期：5
• 全文大小：1,442 KB
• 参考文献：Bartczak D, Nitti S, Millar TM, Kanaras AG (2012) Exocytosis of peptide functionalized gold nanoparticles in endothelial cells. Nanoscale 4(15):4470-472. doi:10.-039/?C2nr31064c View Article
  Boutard T, Rousseau B, Couteau C, Tomasoni C, Simonnard C, Jacquot C, Coiffard LJM, Konstantinov K, Devers T, Roussakis C (2013) Comparison of photoprotection efficiency and antiproliferative activity of ZnO commercial sunscreens and CeO2. Mater Lett 108:13-6. doi:10.-016/?j.?matlet.-013.-6.-85 View Article
  Canton I, Battaglia G (2012) Endocytosis at the nanoscale. Chem Soc Rev 41(7):2718-739. doi:10.-039/?C2cs15309b View Article
  Chaudhry Q, Scotter M, Blackburn J, Ross B, Boxall A, Castle L, Aitken R, Watkins R (2008) Applications and implications of nanotechnologies for the food sector. Food Addit Contam A 25(3):241-58. doi:10.-080/-265203070174453- View Article
  Chen HI, Chang HY (2004) Homogeneous precipitation of cerium dioxide nanoparticles in alcohol/water mixed solvents. Colloids Surf A 242(1-):61-9. doi:10.-016/?j.?colsurfa.-004.-4.-56 View Article
  Chen HI, Chang HY (2005) Synthesis of nanocrystalline cerium oxide particles by the precipitation method. Ceram Int 31(6):795-02. doi:10.-016/?j.?ceramint.-004.-9.-06 View Article
  Chigurupati S, Mughal MR, Okun E, Das S, Kumar A, McCaffery M, Seal S, Mattson MP (2013) Effects of cerium oxide nanoparticles on the growth of keratinocytes, fibroblasts and vascular endothelial cells in cutaneous wound healing. Biomaterials 34(9):2194-201. doi:10.-016/?j.?biomaterials.-012.-1.-61 View Article
  Chithrani BD, Chan WC (2007) Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes. Nano Lett 7(6):1542-550. doi:10.-021/?nl070363y View Article
  Chithrani BD, Ghazani AA, Chan WCW (2006) Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. Nano Lett 6(4):662-68. doi:10.-021/?Nl052396o View Article
  Cho K, Wang X, Nie S, Chen ZG, Shin DM (2008) Therapeutic nanoparticles for drug delivery in cancer. Clin Cancer Res 14(5):1310-316. doi:10.-158/-078-0432.?CCR-07-1441 View Article
  Chu ZQ, Huang YJ, Tao Q, Li Q (2011) Cellular uptake, evolution, and excretion of silica nanoparticles in human cells. Nanoscale 3(8):3291-299. doi:10.-039/?C1nr10499c View Article
  Dombu CY, Kroubi M, Zibouche R, Matran R, Betbeder D (2010) Characterization of endocytosis and exocytosis of cationic nanoparticles in airway epithelium cells. Nanotechnology 21:355102. doi:10.-088/-957-4484/-1/-5/-55102 View Article
  Errington RJ, Brown MR, Silvestre OF, Njoh KL, Chappell SC, Khan IA, Rees P, Wilks SP, Smith PJ, Summers HD (2010) Single cell nanoparticle tracking to model cell cycle dynamics and compartmental inheritance. Cell Cycle 9(1):121-30. doi:10.-161/?Cc.-.-.-0246 View Article
  Hahn MA, Singh AK, Sharma P, Brown SC, Moudgil BM (2011) Nanoparticles as contrast agents for in vivo bioimaging: current status and future perspectives. Anal Bioanal Chem 399(1):3-7. doi:10.-007/?s00216-010-4207-5 View Article
  He B, Jia ZR, Du WW, Yu C, Fan YC, Dai WB, Yuan L, Zhang H, Wang XQ, Wang JC, Zhang X, Zhang Q (2013a) The transport pathways of polymer nanoparticles in MDCK epithelial cells. Biomaterials 34(17):4309-326. doi:10.-016/?j.?biomaterials.-013.-1.-00 View Article
  He B, Lin P, Jia ZR, Du WW, Qu W, Yuan L, Dai WB, Zhang H, Wang XQ, Wang JC, Zhang X, Zhang Q (2013b) The transport mechanisms of polymer nanoparticles in Caco-2 epithelial cells. Biomaterials 34(25):6082-098. doi:10.-016/?j.?biomaterials.-013.-4.-53 View Article
  Hilger I (2013) In vivo applications of magnetic nanoparticle hyperthermia. Int J Hyperth 29(8):828-34. doi:10.-109/-2656736.-013.-32815 View Article
  Hu L, Mao Z, Zhang Y, Gao C (2011) Influences of size of silica particles on the cellular endocytosis, exocytosis and cell activity of HepG2 cells. J Nanosci Lett 1(1):1-6View Article
  Jiang S, Gnanasammandhan MK, Zhang Y (2010) Optical imaging-guided cancer therapy with fluorescent nanoparticles. J R Soc Interface 7(42):3-8. doi:10.-098/?rsif.-009.-243 View Article
  Johannsen M, Gneveckow U, Eckelt L, Feussner A, Wald?fner N, Scholz R, Deger S, Wust P, Loening SA, Jordan A (2005) Clinical hyperthermia of prostate cancer using magnetic nanoparticles: presentation of a new interstitial technique. Int J Hyperth 21(7):637-47. doi:10.-080/-265673050015836- View Article
  Jung H, Kittelson DB, Zachariah MR (2005) The influence of a cerium additive on ultrafine diesel particle emissions and kinetics of oxidation. Combust Flame 142(3):276-88. doi:10.-016/?j.?combustflame.-004.-1.-15 View Article
  Karakoti AS, Monteiro-Riviere NA, Aggarwal R, Davis JP, Narayan RJ, Self WT, McGinnis J, Seal S (2008) Nanoceria as antioxidant: synthesis and biomedical applications. Jom-US 60(3):33-7. doi:10.-007/?s118
• 作者单位：Claudia Strobel (1)
  Hartmut Oehring (2)
  Rudolf Herrmann (3)
  Martin F?rster (4)
  Armin Reller (3)
  Ingrid Hilger (1)
  
  1. Department of Experimental Radiology, Institute of Diagnostic and Interventional Radiology, Jena University Hospital -Friedrich Schiller University Jena, Erlanger Allee 101, 07747, Jena, Germany
  2. Institute of Anatomy II, Jena University Hospital -Friedrich Schiller University Jena, Teichgraben 7, 07743, Jena, Germany
  3. Department of Physics, University of Augsburg, Universitaetsstra?e 1, 86159, Augsburg, Germany
  4. Department of Internal Medicine I, Division of Pulmonary Medicine and Allergy/Immunology, Jena University Hospital -Friedrich Schiller University Jena, Erlanger Allee 101, 07747, Jena, Germany
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Nanotechnology
  Inorganic Chemistry
  Characterization and Evaluation Materials
  Physical Chemistry
  Applied Optics, Optoelectronics and Optical Devices
  
• 出版者：Springer Netherlands
• ISSN：1572-896X

文摘

Although cytotoxicity and endocytosis of nanoparticles have been the subject of numerous studies, investigations regarding exocytosis as an important mechanism to reduce intracellular nanoparticle accumulation are rather rare and there is a distinct lack of knowledge. The current study investigated the behavior of human microvascular endothelial cells to exocytose cerium dioxide (CeO₂) nanoparticles (18.8?nm) by utilization of specific inhibitors [brefeldin A; nocodazole; methyl-β-cyclodextrin (MβcD)] and different analytical methods (flow cytometry, transmission electron microscopy, inductively coupled plasma mass spectrometry). Overall, it was found that endothelial cells were able to release CeO₂ nanoparticles via exocytosis after the migration of nanoparticle containing endosomes toward the plasma membrane. The exocytosis process occurred mainly by fusion of vesicular membranes with plasma membrane resulting in the discharge of vesicular content to extracellular environment. Nevertheless, it seems to be likely that nanoparticles present in the cytosol could leave the cells in a direct manner. MβcD treatment led to the strongest inhibition of the nanoparticle exocytosis indicating a significant role of the plasma membrane cholesterol content in the exocytosis process. Brefeldin A (inhibitor of Golgi-to-cell-surface-transport) caused a higher inhibitory effect on exocytosis than nocodazole (inhibitor of microtubules). Thus, the transfer from distal Golgi compartments to the cell surface influenced the exocytosis process of the CeO₂ nanoparticles more than the microtubule-associated transport. In conclusion, endothelial cells, which came in contact with nanoparticles, e.g., after intravenously applied nano-based drugs, can regulate their intracellular nanoparticle amount, which is necessary to avoid adverse nanoparticle effects on cells.