Two-photon FLIM of NAD(P)H and FAD in mesenchymal stem cells undergoing either osteogenic or chondrogenic differentiation

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• 作者：Aleksandra V. Meleshina ; Varvara V. Dudenkova…
• 关键词：Mesenchymal stem cells ; NAD(P)H ; FAD ; Osteogenic and chondrogenic differentiation ; Metabolic shift ; Two ; photon fluorescence microscopy ; FLIM
• 刊名：Stem Cell Research & Therapy
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：8
• 期：1
• 全文大小：6936KB
• 刊物主题：Stem Cells; Cell Biology;
• 出版者：BioMed Central
• ISSN：1757-6512
• 卷排序：8

文摘

BackgroundMetabolic plasticity and the versatility of different lineages of stem cells as they satisfy their energy demands are not completely understood. In this study we investigated the metabolic changes in mesenchymal stem cells (MSCs) undergoing differentiation in two directions, osteogenic and chondrogenic, using two-photon fluorescence microscopy combined with FLIM.