Fifty-nine microsatellite markers for hybrid classification studies involving endemic Florida Mottled Duck (Anas fulvigula fulvigula) and invasive Mallards (A. platyrhynchos)

详细信息    查看全文

• 作者：Seifu Seyoum (1)
  Michael D. Tringali (1)
  Ronald R. Bielefeld (2)
  Jamie C. Feddersen (3)
  Richard J. Benedict Jr (4)
  Andrew T. Fanning (4)
  Brandon L. Barthel (1)
  Caitlin Curtis (1)
  Cecilia Puchulutegui (1)
  Alicia C. M. Roberts (1)
  Vicki L. Villanova (1)
  Emily C. Tucker (1)
  
• 关键词：Florida Mottled Duck ; Anas fulvigula ; Mallard ; Anas platyrhynchos ; Microsatellite markers ; Hybridization
• 刊名：Conservation Genetics Resources
• 出版年：2012
• 出版时间：September 2012
• 年：2012
• 卷：4
• 期：3
• 页码：681-687
• 全文大小：266KB
• 参考文献：1. Belkhir K, Borsa P, Chikhi L, Raufaste N, Bonhomme F (2000) GENETIX, Windows?software for population genetics. Laboratoire Génome, Populations, Interactions CNRS UMR 5000, Université Montpellier II, Montpellier (France)
  2. Bielefeld RR, Brasher MG, Moorman TE, Gray PN (2010) Mottled Duck ( / Anas fulvigula), The Birds of North America Online (A. Poole, Ed.). Ithaca: Cornell Lab of Ornithology; Retrieved from the Birds of North America Online: http://bna.birds.cornell.edu/bna/species/081
  3. Boecklen WJ, Howard DJ (1997) Genetic analysis of hybrid zones: numbers of markers and power of resolution. Ecology 78:2611-616 CrossRef
  4. Marchuk D, Drumm M, Saulino A, Collins FS (1990) Construction of vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Res 19:1154 CrossRef
  5. McCracken KG, Johnson WP, Sheldon FH (2001) Molecular population genetics, phylogeography, and conservation biology of the Mottled duck ( / Anas fulvigula). Conserv Genet 2:87-02 CrossRef
  6. Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223-25 CrossRef
  7. Rosenberg NA, Li LM, Ward R, Pritchard JK (2003) Informativeness of genetic markers for inference of ancestry. Am J Hum Genet 73:1402-422 CrossRef
  8. Rousset F (2008) Genepop-07: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103-06 CrossRef
  9. Sorensen MD, Tetrault JS, Harrigan RJ (2007) Development of additional genetic markers to differentiate mottled ducks from mallards and hybrids in Florida. Final Report to the Florida Fish and Wildlife Conservation Commission. March 25, 2007. Department of Biology, Boston University, Boston, MA. p 20
  10. St John J, Quinn TW (2008) Rapid capture of DNA targets. Biotechniques 44:259-64 CrossRef
  11. V?h? JP, Primmer CR (2006) Efficiency of model-based Bayesian methods for detecting hybrid individuals under different hybridization scenarios and with different numbers of loci. Mol Ecol 15:63-2 CrossRef
  12. Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535-38 CrossRef
  13. Williams CL, Fedynich AM, Pence DB, Rhodes OE Jr (2005a) Evaluation of allozyme and microsatellite variation in Texas and Florida Mottled ducks. Condor 107:155-61 CrossRef
  14. Williams CL, Brust RC, Fendley TT, Tiller GR Jr, Rhodes OE Jr (2005b) A comparison of hybridization between Mottled duck and Mallard in Florida and South Carolina using microsatellite DNA analysis. Conserv Genet 6:445-53 CrossRef
  
• 作者单位：Seifu Seyoum (1)
  Michael D. Tringali (1)
  Ronald R. Bielefeld (2)
  Jamie C. Feddersen (3)
  Richard J. Benedict Jr (4)
  Andrew T. Fanning (4)
  Brandon L. Barthel (1)
  Caitlin Curtis (1)
  Cecilia Puchulutegui (1)
  Alicia C. M. Roberts (1)
  Vicki L. Villanova (1)
  Emily C. Tucker (1)
  
  1. Florida Fish and Wildlife Conservation Commission, 100 Eighth Avenue S.E., St. Petersburg, FL, 33701, USA
  2. Florida Fish and Wildlife Conservation Commission, 544 Jay Street, Sebastian, FL, 32958, USA
  3. Florida Fish and Wildlife Conservation Commission, 3200 T.M. Goodwin Road, Fellsmere, FL, 32948, USA
  4. Florida Fish and Wildlife Conservation Commission, 8932 Apalachee Parkway, Tallahassee, FL, 32311, USA
  

文摘

Endemic Florida Mottled Duck (Anas fulvigula fulvigula) appear to be hybridizing introgressively with domesticated Mallards (A. platyrhynchos), which are frequently released or escape captivity and have established feral populations throughout Florida. To investigate this possible conservation threat, we isolated and characterized 59 polymorphic loci from an enriched Florida Mottled Duck microsatellite library and performed cross-amplification assays with Mallard specimens. Average numbers of alleles per locus were 6.0 (ranging 2-3) and 5.6 (ranging 2-5) for A. fulvigula and A. platyrhynchos, respectively; estimates of observed/expected heterozygosity were 0.54/0.63 and 0.52/0.64. Markers developed in this study will be used in conjunction with existing markers to robustly classify hybrids and to assess and monitor the genetic dynamics of introgression between these waterfowl species.