Preparation of functionalized Fe₃O₄@SiO₂ magnetic nanoparticles for monoclonal antibody purification

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• 作者：Xuemei Hou ; Changjie Zhao ; Yanlong Tian…
• 关键词：Magnetic ; Fe3O4@SiO2 ; Protein A ; Nanoparticle ; Monoclonal antibody
• 刊名：Chemical Research in Chinese Universities
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：32
• 期：6
• 页码：889-894
• 全文大小：
• 刊物主题：Chemistry/Food Science, general; Analytical Chemistry; Inorganic Chemistry; Organic Chemistry; Physical Chemistry;
• 出版者：Jilin University and The Editorial Department of Chemical Research in Chinese Universities
• ISSN：2210-3171
• 卷排序：32

文摘

Magnetic Fe₃O₄@SiO₂ nanoparticles with superparamagnetic properties were prepared via a reverse microemulsion method at room temperature. The as-prepared samples were characterized by transmission electron microscopy(TEM), X-ray diffractometry(XRD), and vibrating sample magnetometry(VSM). The Fe₃O₄@SiO₂ nanopar-ticles were modified by (3-aminopropyl)triethoxysilane(APTES) and subsequently activated by glutaraldehyde(Glu). Protein A was successfully immobilized covalently onto the Glu activated Fe3O4@SiO2 nanoparticles. The adsorption capacity of the nanoparticles was determined on an ultraviolet spectrophotometer(UV) and approximately up to 203 mg/g of protein A could be uniformly immobilized onto the modified Fe₃O₄@SiO₂ magnetic beads. The core-shell of the Fe₃O₄@SiO₂ magnetic beads decorated with protein A showed a good binding capacity for the chimeric anti-EGFR monoclonal antibody(anti-EGFR mAb). The purity of the anti-EGFR mAb was analyzed by virtue of HPLC. The protein A immobilized affinity beads provided a purity of about 95.4%.