A recombinant porcine circovirus type 2 expressing the VP1 epitope of the type O foot-and-mouth disease virus is infectious and induce both PCV2 and VP1 epitope antibodies

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• 作者：Liping Huang (1)
  Feiyan Zhang (1)
  Qinghai Tang (1)
  Yanwu Wei (1)
  Hongli Wu (1)
  Longjun Guo (1)
  Yujie Fu (1)
  Changming Liu (1)
  
• 关键词：Porcine circovirus type 2 ; Foot ; and ; mouth disease virus ; VP1 ; Neutralizing epitope ; Recombinant virus
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2014
• 出版时间：November 2014
• 年：2014
• 卷：98
• 期：22
• 页码：9339-9350
• 全文大小：2,088 KB
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  14. Huang LP, Luo YH, Wei YW, Guo LJ, Liu CM (2011a) Identification of one critical amino acid that determines a conformational neutralizing epitope in the capsid protein of porcine circovirus type 2. BMC Microbiol 11:188 CrossRef
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• 作者单位：Liping Huang (1)
  Feiyan Zhang (1)
  Qinghai Tang (1)
  Yanwu Wei (1)
  Hongli Wu (1)
  Longjun Guo (1)
  Yujie Fu (1)
  Changming Liu (1)
  
  1. Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin, 150001, People’s Republic of China
  
• ISSN：1432-0614

文摘

Porcine circovirus type 2 (PCV2) is the etiological agent of postweaning multisystemic wasting syndrome, a disease that causes huge economic damage in swine industry. A recombinant PCV2 expressing the neutralizing VP1 epitope (aa 141-60) of the foot-and-mouth disease virus (FMDV) was rescued using an infectious cloning technique. The PCV2 antigen and FMDV-VP1 antigenic epitope of the cloned strain recPCV2-CL-VP1 were confirmed by an immunoperoxidase monolayer assay (IPMA) and a capture enzyme-linked immunosorbent assay (ELISA). The morphological features of the recPCV2-CL-VP1 were not discernibly different from those of its parental strain (PCV2-CL). However, the recombinant virus could be differentiated from its parental virus by PCR and capture ELISA. The recPCV2-CL-VP1 was demonstrated to replicate stably in PK-15 cells through ten passages. An infection experiment using BALB/c mice showed that both recPCV2-CL-VP1 and PCV2-CL could replicate in the mice, cause various pathological changes, and induce a high level of anti-Cap antibodies. The recombinant virus emulsified with Freund’s adjuvant was used to immunize BALB/c mice and induced antibodies against the FMDV-VP1 epitope. Hence, the recombinant PCV2 strain, which expressed the neutralizing FMDV-VP1 epitope, provides a valuable platform to develop novel genetic vaccines.