Resistin Increases Ectopic Deposition of Lipids Through miR-696 in C2C12 Cells

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• 作者：Fengyun Wen ; Haiwei Zhang ; Chen Bao ; Mengshi Yang ; Nan Wang…
• 关键词：Resistin ; Ectopic deposition of lipids ; miR ; 696 ; Skeletal muscle
• 刊名：Biochemical Genetics
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：53
• 期：4-6
• 页码：63-71
• 全文大小：2,912 KB
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• 作者单位：Fengyun Wen (1)
  Haiwei Zhang (1)
  Chen Bao (2)
  Mengshi Yang (1)
  Nan Wang (1)
  Jie Zhang (1)
  Yajie Hu (1)
  Xi Yang (1)
  Jinjing Geng (1)
  Zaiqing Yang (2)
  
  1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471003, Henan, People鈥檚 Republic of China
  2. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People鈥檚 Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Biochemistry
  Zoology
  Medical Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-4927

文摘

Resistin is associated with ectopic deposition of lipids, and determining its developmental and molecular mechanisms may help in the development of novel treatments. MicroRNAs (miRNAs) are involved in many physiological and pathological processes as negative regulators. We performed mouse liver miRNA microarrays to analyze the differences in expression between resistin-treated and control mice; the results showed that miR-696 was significantly upregulated by resistin. Therefore, we aimed to study whether miR-696 played a role in the resistin-induced ectopic deposition of lipids. Quantitative RT-PCR results showed that miR-696 was upregulated both in vivo and in vitro, consistent with the microarray. We transfected C2C12 cells and used miR-696 mimics and inhibitors to assess the role of miR-696 in the resistin-induced ectopic deposition of lipids. The overexpression of miR-696 increased the TG content in C2C12 cells and decreased the mitochondrial content. Next, a study of miR-696's role in the deposition of lipids in C2C12 induced by resistin showed that inhibition of miR-696 restored the TG content by up to 80%, which suggests that, in C2C12 cells, resistin at least partially increases the deposition of lipids through miR-696. miR-696 plays a role in the ectopic deposition of lipids induced by resistin in skeletal muscle at least partially.