Synthesis and characterization of the fluorescent probes for the labeling of Microthrix parvicella
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  • 作者：Songya Li ; Xuening Fei ; Xiumei Jiao ; Dayong Lin…
  • 关键词：Fluorescent probes ; Synthesis ; Characterization ; Labeling ; Microthrix parvicella
  • 刊名：Applied Microbiology and Biotechnology
  • 出版年：2016
  • 出版时间：March 2016
  • 年：2016
  • 卷：100
  • 期：6
  • 页码：2883-2894
  • 全文大小：1,347 KB
  • 参考文献：Andreasen K, Nielsen PH (2000) Growth of Microthrix parvicella in nutrient removal activated sludge plants: studies of in situ physiology. Water Res 34(5):1559–1569CrossRef
    Blackall LL, Seviour EM, Cunningham MA, Seviour RJ, Hugenholtz P (1995) “Microthrix parvicella” is a novel, deep branching member of the actinomycetes subphylum. Syst Appl Microbiol 17(4):513–518CrossRef
    Blackall LL, Stratton H, Bradford D, Del Dot T, SJÖRUP C, Seviour EM, Seviour RJ (1996) “Candidatus Microthrix parvicella”, a filamentous bacterium from activated sludge sewage treatment plants. Int J Syst Bacteriol 46(1):344–346CrossRef PubMed
    Costa J, Mesquita D, Amaral A, Alves M, Ferreira E (2013) Quantitative image analysis for the characterization of microbial aggregates in biological wastewater treatment: a review. Environ Sci Pollut Res 20(9):5887–5912CrossRef
    Daims H, Brühl A, Amann R, Schleifer K-H, Wagner M (1999) The domain-specific probe EUB338 is insufficient for the detection of all bacteria: development and evaluation of a more comprehensive probe set. Syst Appl Microbiol 22(3):434–444CrossRef PubMed
    Dos Santos LA, Ferreira V, Neto MM, Pereira MA, Mota M, Nicolau A (2015) Study of 16 Portuguese activated sludge systems based on filamentous bacteria populations and their relationships with environmental parameters. Appl Microbiol Biotechnol 99(12):5307–5316CrossRef
    Eikelboom D (1975) Filamentous organisms observed in activated sludge. Water Res 9(4):365–388CrossRef
    Eikelboom DH, Van Buijsen HJJ (1983) Microscopic sludge investigation manual. TNO research institute for environmental hygiene. Water and Soil Division, Delft
    Eikelboom DH, Andreadakis A, Andreasen K (1998) Survey of filamentous populations in nutrient removal plants in four European countries. Water Sci Technol 37(4–5):281–289CrossRef
    Erhart R, Bradford D, Seviour RJ, Amann R, Blackall LL (1997) Development and use of fluorescent in situ hybridization probes for the detection and identification of “Microthrix parvicella” in activated sludge. Syst Appl Microbiol 20(2):310–318CrossRef
    Fei X, Gu Y, Li C, Yang X (2012) Study on synthesis and spectrum of novel styryl cyanine dyes with a carbazole bridged chain. J Fluoresc 22(3):807–814CrossRef PubMed
    Fei X, Gu Y, Li C, Liu Y, Yu L (2013) Synthesis and spectra of a kind of novel longer-wavelength benzoxazole indole styryl cyanine dye with a carbazole
    idged chain. J Fluoresc 23(1):221–226CrossRef PubMed
    Grabowski Z, Dobkowski J (1983) Twisted intramolecular charge transfer (TICT) excited states: energy and molecular structure. Pure Appl Chem 55(2):245–252CrossRef
    Guo J, Peng Y, Wang S, Yang X, Yuan Z (2014) Filamentous and non-filamentous bulking of activated sludge encountered under nutrients limitation or deficiency conditions. Chem Eng J 255:453–461CrossRef
    Hamit-Eminovski J, Eskilsson K, Arnebrant T (2010) Change in surface properties of Microthrix parvicella upon addition of polyaluminium chloride as characterized by atomic force microscopy. Biofouling 26(3):323–331CrossRef PubMed
    Jakob Pernthaler FOG, Schönhuber W, Amann R (2001) Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes. Methods in Microbiology. Mar Microbiol 30
    Jenkins D, Richard MG, Daigger GT (2004) Manual on the causes and control of activated sludge bulking, foaming, and other solids separation problems. IWA Publishing, London
    Jon McIlroy S, Kristiansen R, Albertsen M, Michael Karst S, Rossetti S, Lund Nielsen J, Tandoi V, James Seviour R, Nielsen PH (2013) Metabolic model for the filamentous ‘Candidatus Microthrix parvicella’ based on genomic and metagenomic analyses. ISME J 7(6):1161–1172PubMedCentral CrossRef
    Lakowicz JR (2013) Principles of fluorescence spectroscopy. Springer science and business media. USA, New York
    Martins AMP, Pagilla K, Heijnen JJ, van Loosdrecht MCM (2004) Filamentous bulking sludge : a critical review. Water Res 38(4):793–817CrossRef PubMed
    Nielsen J, Mikkelsen L, Nielsen P (2001) In situ detection of cell surface hydrophobicity of probe-defined bacteria in activated sludge. Water Sci Technol 43(6):97–103PubMed
    Nielsen P, Roslev P, Dueholm T, Nielsen J (2002) Microthrix parvicella, a specialized lipid consumer in anaerobic-aerobic activated sludge plants. Water Sci Technol 46(1–2):73–80PubMed
    Noutsopoulos C, Mamais D, Andreadakis A (2010) Long chain fatty acids removal in selector tanks: evidence for insufficient Microthrix parvicella control. Desalin Water Treat 23(1–3):20–25CrossRef
    Noutsopoulos C, Mamais D, Andreadakis A (2012) A hypothesis on Microthrix parvicella proliferation in biological nutrient removal activated sludge systems with selector tanks. FEMS Microbiol Ecol 80(2):380–389CrossRef PubMed
    Rossetti S, Christensson C, Blackall LL, Tandoi V (1997) Phenotypic and phylogenetic description of an Italian isolate of “Microthrix parvicella”. J Appl Microbiol 82(4):405–410CrossRef PubMed
    Rossetti S, Tomei MC, Nielsen PH, Tandoi V (2005) “Microthrix parvicella”, a filamentous bacterium causing bulking and foaming in activated sludge systems: a review of current knowledge. FEMS Microbiol Rev 29(1):49–64CrossRef PubMed
    Salinas-Navarro M, Jiménez-López M, Valiente-Soriano F, Alarcón-Martínez L, Avilés-Trigueros M, Mayor S, Holmes T, Lund R, Villegas-Pérez M, Vidal-Sanz M (2009) Retinal ganglion cell population in adult albino and pigmented mice: a computerized analysis of the entire population and its spatial distribution. Vis Res 49(6):637–647CrossRef PubMed
    Sanz JL, Köchling T (2007) Molecular biology techniques used in wastewater treatment: an overview. Process Biochem 42(2):119–133CrossRef
    Sarkar N, Das K, Nath DN, Bhattacharyya K (1994) Twisted charge transfer processes of Nile red in homogeneous solutions and in faujasite zeolite. Langmuir 10(1):326–329CrossRef
    Seviour E, Williams C, DeGrey B, Soddell J, Seviour R, Lindrea K (1994) Studies on filamentous bacteria from Australian activated sludge plants. Water Res 28(11):2335–2342CrossRef
    Slijkhuis H (1983) Microthrix parvicella, a filamentous bacterium isolated from activated sludge: cultivation in a chemically defined medium. Appl Environ Microbiol 46(4):832–839PubMedCentral PubMed
    Slijkhuis H, Deinema M (1988) Effect of environmental conditions on the occurrence of Microthrix parvicella in activated sludge. Water Res 22(7):825–828CrossRef
    Van Veen WL (1973) Bacteriology of activated sludge, in particular the filamentous bacteria. Antonie Van Leeuwenhoek 39(2):189–205CrossRef PubMed
    Vanysacker L, Denis C, Roels J, Verhaeghe K, Vankelecom IFJ (2014) Development and evaluation of a TaqMan duplex real-time PCR quantification method for reliable enumeration of Candidatus Microthrix. J Microbiol Methods 97:6–14CrossRef PubMed
    Wagner M, Horn M, Daims H (2003) Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes. Curr Opin Microbiol 6(3):302–309CrossRef PubMed
    Wágner DS, Ramin E, Szabo P, Dechesne A, Plósz BG (2015) Microthrix parvicella abundance associates with activated sludge settling velocity and rheology–quantifying and modelling filamentous bulking. Water Res 78:121–132CrossRef PubMed
    Wang J, Qi R, Liu M, Li Q, Bao H, Li Y, Wang S, Tandoi V, Yang M (2014) The potential role of ‘Candidatus Microthrix parvicella’ in phosphorus removal during sludge bulking in two full-scale enhanced biological phosphorus removal plants. Water Sci Technol 70(2):367–375CrossRef PubMed
    Yu L, Li L, Fei X (2014) Spectral study, stability and protein labeling of two carbazole–benzothiazole derivatives. J Lumin 149:28–34CrossRef
  • 作者单位：Songya Li (1)
    Xuening Fei (1) (2) (3)
    Xiumei Jiao (1) (3)
    Dayong Lin (3)
    Baolian Zhang (2)
    Lingyun Cao (2)

    1. School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin, 300384, China
    2. School of Science, Tianjin Chengjian University, Tianjin, 300384, China
    3. School of Environmental Science and Engineering, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin, 300072, China
  • 刊物类别：Chemistry and Materials Science
  • 刊物主题：Chemistry
    Biotechnology
    Microbiology
    Microbial Genetics and Genomics
  • 出版者：Springer Berlin / Heidelberg
  • ISSN：1432-0614
文摘
Although the fluorescent in situ hybridization (FISH) has been widely used to identify the Microthrix parvicella (M. parvicella), there are a few disadvantages and difficulties, such as complicated process, time consuming, etc. In this work, a series of fluorescent probes, which were modified by long-chain alkane with hydrophobic property and based on the property of M. parvicella utilizing long-chain fatty acids (LCFA), for the labeling of M. parvicella in bulking sludge were designed, synthesized, and characterized. The probes were characterized by ultraviolet–visible (UV–Vis) absorption spectra, fluorescence spectra, 1H NMR spectra, and mass spectra, and the photostability and hydrophobic property of probes were investigated. All the results showed that the probes were quite stable and suitable for the fluorescent labeling. The probes had a large stoke shift of 98–137 nm, which was benefit for the fluorescent labeling. In the fluorescent labeling of M. parvicella by the synthesized probes, the probes had excellent labeling effects. By comparison of the images and the Image Pro Plus 6.0 analysis, the optimal concentration of the probes in the activated sludge sample for labeling was 0.010 mmol/L and the probe 3d had the best labeling. In addition, the effect of the duration time of probes was also investigated, and the results showed that the fluorescent intensity of probes hardly changed in a long period of time and it was suitable for labeling.
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