Universal modular system for in vitro screening of potential inhibitors of HIV-1 replication

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• 作者：M. M. Prokofjeva (1)
  N. N. Orlova (1) (2)
  A. S. Gornostaeva (1) (2)
  A. A. Shulgin (1) (2)
  N. A. Nikitenko (1)
  V. N. Senchenko (1)
  T. D. Lebedev (1)
  P. V. Spirin (1)
  K. Riecken (3)
  B. Fehse (3)
  C. Stocking (4)
  V. S. Prassolov (1) (2)
  
• 关键词：HIV ; pseudo ; HIV ; 1 particles ; reverse transcriptase ; nucleoside inhibitors ; non ; nucleoside inhibitors ; integrase inhibitors ; sulfated polysaccharides
• 刊名：Molecular Biology
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：48
• 期：2
• 页码：297-300
• 全文大小：
• 参考文献：1. Barre-Sinoussi F., Chermann J.C., Rey F., Nugeyre M.T., Chamaret S., Gruest J., Dauguet C., Axler-Blin C., V茅zinet-Brun F., Rouzioux C., Rozenbaum W., Montagnier L. 1983. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). / Science. 220, 868鈥?71. CrossRef
  2. Popovic M., Sarngadharan M.G., Read E., Gallo R.C. 1984. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. / Science. 224(4648), 497鈥?00. CrossRef
  3. De Clercq E. 2009. The history of antiretrovirals: Key discoveries over the past 25 years. / Rev. Med. Virol. 19, 287鈥?99. CrossRef
  4. Weber K., Bartsch U., Stocking C., Fehse B. 2008. A multi-color panel of novel lentiviral 鈥済ene ontology鈥?(LeGO) vectors for functional gene analysis. / Mol. Ther. 16(4), 698鈥?06. CrossRef
  5. Prokofjeva M.M., Riecken K., Spirin P.V., Yanvar茅v D.V., D眉sedau A., Ellinger B., Fehse B., Stocking C., Prassolov V.S. 2013. A new system for parallel drug screening against multiple-resistant HIV mutants based on lentiviral self-inactivating (SIN) vectors and multi-colour analyses. / AIDS Research and Therapy. 10(1), 1. CrossRef
  6. Prokofjeva M.M., Spirin P.V., Yanvarev D.V., Ivanov A.V., Novikov M.S., Stepanov O.A., Gottikh M.B., Kochetkov S.N., Fehse B., Stocking C., Prassolov V.S. 2011. Screening of potential HIV-1 inhibitors/replication blockers using secure lentiviral in vitro system. / Acta Naturae. 3, 55鈥?5.
  7. Stepanov O. A., Prokof鈥檈va M.M., Stocking C., Varlamov V.P., Levov A.N., Vikhoreva G.A., Spirin P.V., Mikhailov S.N., Prassolov V.S. 2012. Replicationcompetent gamma-retrovirus Mo-MuLV expressing green fluorescent protein as efficient tool for screening of inhibitors of retroviruses that use heparan sulfate as primary cell receptor. / Mol. Biol. (Moscow). 46(3), 457鈥?66. CrossRef
  8. Prokofjeva M.M., Imbs T.I., Shevchenko N.M., Spirin P.V., Horn S., Fehse B., Zvyagintseva T.N., Prassolov V.S. 2013. Fucoidans as potential inhibitors of HIV-1. / Marine Drugs. 11(8), 3000鈥?014. CrossRef
  
• 作者单位：M. M. Prokofjeva (1)
  N. N. Orlova (1) (2)
  A. S. Gornostaeva (1) (2)
  A. A. Shulgin (1) (2)
  N. A. Nikitenko (1)
  V. N. Senchenko (1)
  T. D. Lebedev (1)
  P. V. Spirin (1)
  K. Riecken (3)
  B. Fehse (3)
  C. Stocking (4)
  V. S. Prassolov (1) (2)
  
  1. Engelhardt Institute of Molecular Biology, Moscow, 119991, Russia
  2. Moscow Institute of Physics and Technology (State University), Dolgoprudny, Moscow Region, 141700, Russia
  3. Research Dept. Cell & Gene Therapy, University Medical Center Hamburg-Eppendorf, D-20246, Hamburg, Germany
  4. Heinrich Pette Institute for Experimental Virology and Immunology, Hamburg, D-20251, Germany
  
• ISSN：1608-3245

文摘

A system based on recombinant lentiviral vectors has been designed for safe screening of potential anti-HIV drugs. The system can be used to evaluate the sensitivity of HIV-1 reverse transcriptase and integrase (wild-type as well as mutant forms of these enzymes detected in drug-resistant virus isolates) to different drugs and substances, as well as to screen inhibitors of other stages of the HIV-1 life cycle.