Properties of sodium currents in neonatal and young adult mouse superficial dorsal horn neurons

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• 作者：Melissa A Tadros ; Kristen E Farrell ; Brett A Graham ; Alan M Brichta…
• 关键词：Development ; Activation ; Spinal cord ; Pain ; Action potential
• 刊名：Molecular Pain
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：11
• 期：1
• 全文大小：5,490 KB
• 参考文献：1. Walsh, MA, Graham, BA, Brichta, AM, Callister, RJ (2009) Evidence for a critical period in the development of excitability and potassium currents in mouse lumbar superficial dorsal horn neurons. J Neurophysiol 101: pp. 1800-12 external" href="http://dx.doi.org/10.1152/jn.90755.2008" target="_blank" title="It opens in new window">CrossRef
  2. Goldin, AL, Barchi, RL, Caldwell, JH, Hofmann, F, Howe, JR, Hunter, JC (2000) Nomenclature of voltage-gated sodium channels. Neuron 28: pp. 365-8 external" href="http://dx.doi.org/10.1016/S0896-6273(00)00116-1" target="_blank" title="It opens in new window">CrossRef
  3. Fukuoka, T, Kobayashi, K, Noguchi, K (2010) Laminae-specific distribution of alpha-subunits of voltage-gated sodium channels in the adult rat spinal cord. Neuroscience 169: pp. 994-1006 external" href="http://dx.doi.org/10.1016/j.neuroscience.2010.05.058" target="_blank" title="It opens in new window">CrossRef
  4. Hildebrand, ME, Mezeyova, J, Smith, PL, Salter, MW, Tringham, E, Snutch, TP (2011) Identification of sodium channel isoforms that mediate action potential firing in lamina I/II spinal cord neurons. Mol Pain 7: pp. 67 external" href="http://dx.doi.org/10.1186/1744-8069-7-67" target="_blank" title="It opens in new window">CrossRef
  5. Beckh, S, Noda, M, Lübbert, H, Numa, S (1989) Differential regulation of three sodium channel messenger RNAs in the rat central nervous system during development. EMBO J 8: pp. 3611-6
  6. Felts, PA, Yokoyama, S, Dib-Hajj, S, Black, JA, Waxman, SG (1997) Sodium channel alpha-subunit mRNAs I, II, III, NaG, Na6 and hNE (PN1): different expression patterns in developing rat nervous system. Brain Res Mol Brain Res 45: pp. 71-82 external" href="http://dx.doi.org/10.1016/S0169-328X(96)00241-0" target="_blank" title="It opens in new window">CrossRef
  7. Blankenship, ML, Coyle, DE, Baccei, ML (2013) Transcriptional expression of voltage-gated Na(+) and voltage-independent K(+) channels in the developing rat superficial dorsal horn. Neuroscience 231: pp. 305-14 external" href="http://dx.doi.org/10.1016/j.neuroscience.2012.11.053" target="_blank" title="It opens in new window">CrossRef
  8. Hughes, DI, Sikander, S, Kinnon, CM, Boyle, KA, Watanabe, M, Callister, RJ (2012) Morphological, neurochemical and electrophysiological features of parvalbumin-expressing cells: a likely source of axo-axonic inputs in the mouse spinal dorsal horn. J Physiol 590: pp. 3927-51 external" href="http://dx.doi.org/10.1113/jphysiol.2012.235655" target="_blank" title="It opens in new window">CrossRef
  9. Yasaka, T, Tiong, SY, Hughes, DI, Riddell, JS, Todd, AJ (2010) Populations of inhibitory and excitatory interneurons in lamina II of the adult rat spinal dorsal horn revealed by a combined electrophysiological and anatomical approach. Pain 151: pp. 475-88 external" href="http://dx.doi.org/10.1016/j.pain.2010.08.008" target="_blank" title="It opens in new window">CrossRef
  10. Safronov, BV (1999) Spatial distribution of Na-?and K+ channels in spinal dorsal horn neurones: role of the soma, axon and dendrites in spike generation. Prog Neurobiol 59: pp. 217-41 external" href="http://dx.doi.org/10.1016/S0301-0082(98)00051-3" target="_blank" title="It opens in new window">CrossRef
  11. Safronov, BV, Wolff, M, Vogel, W (1999) Axonal expression of sodium channels in rat spinal neurones during postnatal development. J Physiol (Lond) 514: pp. 729-34 external" href="http://dx.doi.org/10.1111/j.1469-7793.1999.729ad.x" target="_blank" title="It opens in new window">CrossRef
  12. Li, J, Baccei, ML (2011) Pacemaker neurons within newborn spinal pain circuits. J Neurosci 31: pp. 9010-22 external" href="http://dx.doi.org/10.1523/JNEUROSCI.6555-10.2011" target="_blank" title="It opens in new window">CrossRef
  13. Shatz, CJ, Stryker, MP (1988) Prenatal tetrodotoxin infusion blocks segregation of retinogeniculate afferents. Science 242: pp. 87-9 external" href="http://dx.doi.org/10.1126/science.3175636" target="_blank" title="It opens in new window">CrossRef
  14. Cummins, TR, Aglieco, F, Renganathan, M, Herzog, RI, Dib-Hajj, SD, Waxman, SG (2001) Nav1.3 sodium channels: rapid repriming and slow closed-state inactivation display quantitative differences after expression in a mammalian cell line and in spinal sensory neurons. J Neurosci 21: pp. 5952-61
  15. Choi, JS, Dib-Hajj, SD, Waxman, SG (2007) Differential slow inactivation and use-dependent inhibition of Nav1.8 channels contribute to distinct firing properties in IB4+ and IB4- DRG neurons. J Neurophysiol 97: pp. 1258-65 external" href="http://dx.doi.org/10.1152/jn.01033.2006" target="_blank" title="It opens in new window">CrossRef
  16. Graham, BA, Callister, RJ Pain. In: editors">Watson, CGPL eds. (2011) The Mou
• 刊物主题：Pain Medicine; Molecular Medicine;
• 出版者：BioMed Central
• ISSN：1744-8069

文摘

Background Superficial dorsal horn (SDH) neurons process nociceptive information and their excitability is partly determined by the properties of voltage-gated sodium channels. Recently, we showed the excitability and action potential properties of mouse SDH neurons change markedly during early postnatal development. Here we compare sodium currents generated in neonate (P0-5) and young adult (≥P21) SDH neurons. Results Whole cell recordings were obtained from lumbar SDH neurons in transverse spinal cord slices (CsF internal, 32°C). Fast activating and inactivating TTX-sensitive inward currents were evoked by depolarization from a holding potential of ?00?mV. Poorly clamped currents, based on a deflection in the IV relationship at potentials between ?0 and ?0?mV, were not accepted for analysis. Current density and decay time increased significantly between the first and third weeks of postnatal development, whereas time to peak was similar at both ages. This was accompanied by more subtle changes in activation range and steady state inactivation. Recovery from inactivation was slower and TTX-sensitivity was reduced in young adult neurons. Conclusions Our study suggests sodium channel expression changes markedly during early postnatal development in mouse SDH neurons. The methods employed in this study can now be applied to future investigations of spinal cord sodium channel plasticity in murine pain models.