RNA-seq transcriptional profiling of Herbaspirillum seropedicae colonizing wheat (Triticum aestivum) roots

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RNA-seq transcriptional profiling of Herbaspirillum seropedicae colonizing wheat (Triticum aestivum) roots

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作者：V. C. S. Pankievicz ; D. Camilios-Neto ; P. Bonato ; E. Balsanelli…
关键词：Biological nitrogen fixation ; H. seropedicae ; RNA ; seq profiling ; Rhizosphere ; PGPB ; plant growth promoting bacteria
刊名：Plant Molecular Biology
出版年：2016
出版时间：April 2016
年：2016
卷：90
期：6
页码：589-603
全文大小：1,057 KB
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作者单位：V. C. S. Pankievicz (1)
D. Camilios-Neto (3)
P. Bonato (1)
E. Balsanelli (1)
M. Z. Tadra-Sfeir (1)
H. Faoro (1)
L. S. Chubatsu (1)
L. Donatti (2)
G. Wajnberg (4) (5)
F. Passetti (4) (5)
R. A. Monteiro (1)
F. O. Pedrosa (1)
E. M. Souza (1)

1. Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil
3. Department of Biochemistry and Biotechnology, Universidade Estadual de Londrina, Londrina, PR, Brazil
2. Department of Cellular Biology, Universidade Federal do Paraná, Curitiba, PR, Brazil
4. Bioinformatics Unit, Clinical Research Coordination, Instituto Nacional de Câncer, Rio de Janeiro, RJ, Brazil
5. Laboratory of Functional Genomics and Bioinformatics, Oswaldo Cruz Institute, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, RJ, Brazil
刊物类别：Biomedical and Life Sciences
刊物主题：Life Sciences
Plant Sciences
Biochemistry
Plant Pathology
出版者：Springer Netherlands
ISSN：1573-5028

文摘

Herbaspirillum seropedicae is a diazotrophic and endophytic bacterium that associates with economically important grasses promoting plant growth and increasing productivity. To identify genes related to bacterial ability to colonize plants, wheat seedlings growing hydroponically in Hoagland’s medium were inoculated with H. seropedicae and incubated for 3 days. Total mRNA from the bacteria present in the root surface and in the plant medium were purified, depleted from rRNA and used for RNA-seq profiling. RT-qPCR analyses were conducted to confirm regulation of selected genes. Comparison of RNA profile of root attached and planktonic bacteria revealed extensive metabolic adaptations to the epiphytic life style. These adaptations include expression of specific adhesins and cell wall re-modeling to attach to the root. Additionally, the metabolism was adapted to the microxic environment and nitrogen-fixation genes were expressed. Polyhydroxybutyrate (PHB) synthesis was activated, and PHB granules were stored as observed by microscopy. Genes related to plant growth promotion, such as auxin production were expressed. Many ABC transporter genes were regulated in the bacteria attached to the roots. The results provide new insights into the adaptation of H. seropedicae to the interaction with the plant.