Filifactor alocis - involvement in periodontal biofilms

详细信息    查看全文

• 作者：Sebastian Schlafer (1)
  Birgit Riep (2)
  Ann L Griffen (3)
  Annett Petrich (1)
  Julia Hübner (1)
  Moritz Berning (1)
  Anton Friedmann (2)
  Ulf B G?bel (1)
  Annette Moter (1)
  
• 刊名：BMC Microbiology
• 出版年：2010
• 出版时间：December 2010
• 年：2010
• 卷：10
• 期：1
• 全文大小：2211KB
• 参考文献：1. Haffajee AD, Socransky SS: Microbial etiological agents of destructive periodontal diseases. / Periodontol 2000 1994, 5:78-11. CrossRef
  2. Kolenbrander PE, London J: Adhere today, here tomorrow: oral bacterial adherence. / J Bacteriol 1993, 175:3247-252.
  3. Dahlen GG: Black-pigmented gram-negative anaerobes in periodontitis. / FEMS Immunol Med Microbiol 1993, 6:181-92. CrossRef
  4. Fives-Taylor PM, Meyer DH, Mintz KP, Brissette C: Virulence factors of Actinobacillus actinomycetemcomitans. / Periodontol 2000 1999, 20:136-67. CrossRef
  5. Cutler CW, Kalmar JR, Genco CA: Pathogenic strategies of the oral anaerobe, Porphyromonas gingivalis. / Trends Microbiol 1995, 3:45-1. CrossRef
  6. Sela MN: Role of Treponema denticola in periodontal diseases. / Crit Rev Oral Biol Med 2001, 12:399-13. CrossRef
  7. Slots J, Listgarten MA: Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal diseases. / J Clin Periodontol 1988, 15:85-3. CrossRef
  8. Murray PA, French CK: DNA probe detection of periodontal pathogens. In / New biotechnology in oral research. Edited by: WM M. Basel: Karger; 1989:33-3.
  9. Chuba PJ, Pelz K, Krekeler G, de Isele TS, Gobel U: Synthetic oligodeoxynucleotide probes for the rapid detection of bacteria associated with human periodontitis. / J Gen Microbiol 1988, 134:1931-938.
  10. Conrads G: DNA probes and primers in dental practice. / Clin Infect Dis 2002, 35:S72-7. CrossRef
  11. Dymock D, Weightman AJ, Scully C, Wade WG: Molecular analysis of microflora associated with dentoalveolar abscesses. / J Clin Microbiol 1996, 34:537-42.
  12. Kroes I, Lepp PW, Relman DA: Bacterial diversity within the human subgingival crevice. / Proc Natl Acad Sci USA 1999, 96:14547-4552. CrossRef
  13. Sakamoto M, Umeda M, Ishikawa I, Benno Y: Comparison of the oral bacterial flora in saliva from a healthy subject and two periodontitis patients by sequence analysis of 16S rDNA libraries. / Microbiol Immunol 2000, 44:643-52.
  14. Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN, Levanos VA, Sahasrabudhe A, Dewhirst FE: Bacterial diversity in human subgingival plaque. / J Bacteriol 2001, 183:3770-783. CrossRef
  15. Paster BJ, Falkler WA Jr, Enwonwu CO, Idigbe EO, Savage KO, Levanos VA, Tamer MA, Ericson RL, Lau CN, Dewhirst FE: Prevalent bacterial species and novel phylotypes in advanced noma lesions. / J Clin Microbiol 2002, 40:2187-191. CrossRef
  16. Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE: Defining the normal bacterial flora of the oral cavity. / J Clin Microbiol 2005, 43:5721-732. CrossRef
  17. Becker MR, Paster BJ, Leys EJ, Moeschberger ML, Kenyon SG, Galvin JL, Boches SK, Dewhirst FE, Griffen AL: Molecular analysis of bacterial species associated with childhood caries. / J Clin Microbiol 2002, 40:1001-009. CrossRef
  18. Kumar PS, Griffen AL, Moeschberger ML, Leys EJ: Identification of candidate periodontal pathogens and beneficial species by quantitative 16S clonal analysis. / J Clin Microbiol 2005, 43:3944-955. CrossRef
  19. Kumar PS, Leys EJ, Bryk JM, Martinez FJ, Moeschberger ML, Griffen AL: Changes in periodontal health status are associated with bacterial community shifts as assessed by quantitative 16S cloning and sequencing. / J Clin Microbiol 2006, 44:3665-673. CrossRef
  20. Riep B, Edesi-Neuss L, Claessen F, Skarabis H, Ehmke B, Flemmig TF, Bernimoulin JP, Gobel UB, Moter A: Are putative periodontal pathogens reliable diagnostic markers? / J Clin Microbiol 2009, 47:1705-711. CrossRef
  21. Donlan RM, Costerton JW: Biofilms: survival mechanisms of clinically relevant microorganisms. / Clin Microbiol Rev 2002, 15:167-93. CrossRef
  22. Cato EP, Moore LVH, Moore WEC: Fusobacterium alocis sp. nov. and Fusobacterium sulci sp. nov. from the human gingival sulcus. / Int J Syst Bacteriol 1985, 35:475-77. CrossRef
  23. Jalava J, Eerola E: Phylogenetic analysis of Fusobacterium alocis and Fusobacterium sulci based on 16S rRNA gene sequences: proposal of Filifactor alocis (Cato, Moore and Moore) comb. nov. and Eubacterium sulci (Cato, Moore and Moore) comb. nov. / Int J Syst Bacteriol 1999,49(Pt 4):1375-379. CrossRef
  24. Maiden MF, Tanner A, Macuch PJ: Rapid characterization of periodontal bacterial isolates by using fluorogenic substrate tests. / J Clin Microbiol 1996, 34:376-84.
  25. Ohta K, Makinen KK, Loesche WJ: Purification and characterization of an enzyme produced by Treponema denticola capable of hydrolyzing synthetic trypsin substrates. / Infect Immun 1986, 53:213-20.
  26. Loesche WJ: The identification of bacteria associated with periodontal disease and dental caries by enzymatic methods. / Oral Microbiol Immunol 1986, 1:65-2. CrossRef
  27. Kumar PS, Griffen AL, Barton JA, Paster BJ, Moeschberger ML, Leys EJ: New bacterial species associated with chronic periodontitis. / J Dent Res 2003, 82:338-44. CrossRef
  28. Dahlen G, Leonhardt A: A new checkerboard panel for testing bacterial markers in periodontal disease. / Oral Microbiol Immunol 2006, 21:6-1. CrossRef
  29. Hutter G, Schlagenhauf U, Valenza G, Horn M, Burgemeister S, Claus H, Vogel U: Molecular analysis of bacteria in periodontitis: evaluation of clone libraries, novel phylotypes and putative pathogens. / Microbiology 2003, 149:67-5. CrossRef
  30. Siqueira JF Jr, Rocas IN: Detection of Filifactor alocis in endodontic infections associated with different forms of periradicular diseases. / Oral Microbiol Immunol 2003, 18:263-65. CrossRef
  31. Wecke J, Kersten T, Madela K, Moter A, Gobel UB, Friedmann A, Bernimoulin J: A novel technique for monitoring the development of bacterial biofilms in human periodontal pockets. / FEMS Microbiol Lett 2000, 191:95-01. CrossRef
  32. Maidak BL, Cole JR, Lilburn TG, Parker CT Jr, Saxman PR, Farris RJ, Garrity GM, Olsen GJ, Schmidt TM, Tiedje JM: The RDP-II (Ribosomal Database Project). / Nucleic Acids Res 2001, 29:173-74. CrossRef
  33. Amann RI, Binder BJ, Olson RJ, Chisholm SW, Devereux R, Stahl DA: Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. / Appl Environ Microbiol 1990, 56:1919-925.
  34. Loy A, Horn M, Wagner M: probeBase: an online resource for rRNA-targeted oligonucleotide probes. / Nucleic Acids Res 2003, 31:514-16. CrossRef
  35. Armitage GC: Development of a classification system for periodontal diseases and conditions. / Northwest Dent 2000, 79:31-5.
  36. Syed SA, Loesche WJ: Survival of human dental plaque flora in various transport media. / Appl Microbiol 1972, 24:638-44.
  37. Moter A, Hoenig C, Choi BK, Riep B, Gobel UB: Molecular epidemiology of oral treponemes associated with periodontal disease. / J Clin Microbiol 1998, 36:1399-403.
  38. Moter A, Leist G, Rudolph R, Schrank K, Choi BK, Wagner M, Gobel UB: Fluorescence in situ hybridization shows spatial distribution of as yet uncultured treponemes in biopsies from digital dermatitis lesions. / Microbiology 1998,144(Pt 9):2459-467. CrossRef
  39. Schlafer S, Nordhoff M, Wyss C, Strub S, Hubner J, Gescher DM, Petrich A, Gobel UB, Moter A: Involvement of Guggenheimella bovis in digital dermatitis lesions of dairy cows. / Vet Microbiol 2008, 128:118-25. CrossRef
  40. Sunde PT, Olsen I, Gobel UB, Theegarten D, Winter S, Debelian GJ, Tronstad L, Moter A: Fluorescence in situ hybridization (FISH) for direct visualization of bacteria in periapical lesions of asymptomatic root-filled teeth. / Microbiology 2003, 149:1095-102. CrossRef
  41. Daims H, Lucker S, Wagner M: daime, a novel image analysis program for microbial ecology and biofilm research. / Environ Microbiol 2006, 8:200-13. CrossRef
  42. ten Cate JM: Biofilms, a new approach to the microbiology of dental plaque. / Odontology 2006, 94:1-. CrossRef
  43. Listgarten MA: Structure of the microbial flora associated with periodontal health and disease in man. A light and electron microscopic study. / J Periodontol 1976, 47:1-8. CrossRef
  44. Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ, Dymock D, Wade WG: Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. / Appl Environ Microbiol 1998, 64:795-99.
  45. Rickard AH, Gilbert P, High NJ, Kolenbrander PE, Handley PS: Bacterial coaggregation: an integral process in the development of multi-species biofilms. / Trends Microbiol 2003, 11:94-00. CrossRef
  
• 作者单位：Sebastian Schlafer (1)
  Birgit Riep (2)
  Ann L Griffen (3)
  Annett Petrich (1)
  Julia Hübner (1)
  Moritz Berning (1)
  Anton Friedmann (2)
  Ulf B G?bel (1)
  Annette Moter (1)
  
  1. Institut für Mikrobiologie und Hygiene, Charité - Universit?tsmedizin Berlin, Dorotheenstra?e 96, 10117, Berlin, Germany
  2. Abteilung Parodontologie, Centrum für Zahn-, Mund- und Kieferheilkunde, Charité - Universit?tsmedizin Berlin, A?mannhauser Stra?e 4-6, 14197, Berlin, Germany
  3. Section of Pediatric Dentistry, College of Dentistry, Ohio State University, 305 W. 12th Avenue, 43210, Columbus, Ohio, USA
  
• ISSN：1471-2180

文摘

Background Bacteria in periodontal pockets develop complex sessile communities that attach to the tooth surface. These highly dynamic microfloral environments challenge both clinicians and researchers alike. The exploration of structural organisation and bacterial interactions within these biofilms is critically important for a thorough understanding of periodontal disease. In recent years, Filifactor alocis, a fastidious, Gram-positive, obligately anaerobic rod was repeatedly identified in periodontal lesions using DNA-based methods. It has been suggested to be a marker for periodontal deterioration. The present study investigated the epidemiology of F. alocis in periodontal pockets and analysed the spatial arrangement and architectural role of the organism in in vivo grown subgingival biofilms. Results A species-specific oligonucleotide probe, FIAL, was designed and evaluated. A total of 490 subgingival plaque samples were submitted to PCR and subsequent dot blot hybridization to compare the prevalence of F. alocis in patients suffering from generalized aggressive periodontitis (GAP), chronic periodontitis (CP), and control subjects resistant to periodontitis. Moreover, a specially designed carrier system was used to collect in vivo grown subgingival biofilms from GAP patients. Subsequent topographic analysis was performed using fluorescence in situ hybridization. While the majority of patients suffering from GAP or CP harboured F. alocis, it was rarely detected in the control group. In the examined carrier-borne biofilms the organism predominantly colonized apical parts of the pocket in close proximity to the soft tissues and was involved in numerous structures that constitute characteristic architectural features of subgingival periodontal biofilms. Conclusions F. alocis is likely to make a relevant contribution to the pathogenetic structure of biofilms accounting for periodontal inflammation and can be considered an excellent marker organism for periodontal disease.