NMR resonance assignments for the class II GTP binding RNA aptamer in complex with GTP

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• 作者：Antje C. Wolter ; Elke Duchardt-Ferner ; Amir H. Nasiri…
• 关键词：RNA ; NMR ; assignment ; Triple resonance experiments ; Aptamer ; GTP ; Selective labeling
• 刊名：Biomolecular NMR Assignments
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：10
• 期：1
• 页码：101-105
• 全文大小：556 KB
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• 作者单位：Antje C. Wolter (1) (2)
  Elke Duchardt-Ferner (1) (2)
  Amir H. Nasiri (1) (2)
  Katharina Hantke (1) (2)
  Christoph H. Wunderlich (3)
  Christoph Kreutz (3)
  Jens Wöhnert (1) (2)
  
  1. Institute for Molecular Biosciences, Johann-Wolfgang-Goethe-University, Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany
  2. Center for Biomolecular Magnetic Resonance (BMRZ), Johann-Wolfgang-Goethe-University, Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany
  3. Institute of Organic Chemistry, Centre for Molecular Biosciences (CMBI), University of Innsbruck, Innrain 80/82, 6020, Innsbruck, Austria
  
• 刊物类别：Physics and Astronomy
• 刊物主题：None Assigned
• 出版者：Springer Netherlands
• ISSN：1874-270X

文摘

The structures of RNA-aptamer-ligand complexes solved in the last two decades were instrumental in realizing the amazing potential of RNA for forming complex tertiary structures and for molecular recognition of small molecules. For GTP as ligand the sequences and secondary structures for multiple families of aptamers were reported which differ widely in their structural complexity, ligand affinity and ligand functional groups involved in RNA-binding. However, for only one of these families the structure of the GTP-RNA complex was solved. In order to gain further insights into the variability of ligand recognition modes we are currently determining the structure of another GTP-aptamer—the so-called class II aptamer—bound to GTP using NMR-spectroscopy in solution. As a prerequisite for a full structure determination, we report here 1H, 13C, 15N and partial 31P-NMR resonance assignments for the class II GTP-aptamer bound to GTP.