Optimization of heterologous DNA-prime, protein boost regimens and site of vaccination to enhance therapeutic immunity against human papillomavirus-associated disease

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• 作者：Shiwen Peng ; Jin Qiu ; Andrew Yang ; Benjamin Yang ; Jessica Jeang…
• 关键词：Human papilloma virus ; HPV ; TA ; CIN ; pNGVL4a ; Sig/E7(detox)/HSP70 ; pNGVL4a ; CRT/E7(detox) ; pNGVL4a ; CRT ; E6E7L2 ; Prime ; boost
• 刊名：Cell & Bioscience
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：6
• 期：1
• 全文大小：1,923 KB
• 刊物主题：Cell Biology; Microbiology;
• 出版者：BioMed Central
• ISSN：2045-3701

文摘

Background Human papillomavirus (HPV) has been identified as the primary etiologic factor of cervical cancer as well as subsets of anogenital and oropharyngeal cancers. The two HPV viral oncoproteins, E6 and E7, are uniquely and consistently expressed in all HPV infected cells and are therefore promising targets for therapeutic vaccination. Both recombinant naked DNA and protein-based HPV vaccines have been demonstrated to elicit HPV-specific CD8+ T cell responses that provide therapeutic effects against HPV-associated tumor models. Here we examine the immunogenicity in a preclinical model of priming with HPV DNA vaccine followed by boosting with filterable aggregates of HPV 16 L2E6E7 fusion protein (TA-CIN).