Effects of the physicochemical properties of titanium dioxide nanoparticles, commonly used as sun protection agents, on microvascular endothelial cells

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• 作者：Claudia Strobel (1)
  Adriano A. Torrano (2)
  Rudolf Herrmann (3)
  Marcelina Malissek (4)
  Christoph Bruchle (2)
  Armin Reller (3)
  Lennart Treuel (4) (5) (6)
  Ingrid Hilger (1)
  
• 关键词：Endothelial cells ; Environmental and health effects ; Nanoparticle ; Nanotoxicology ; Sun protection agent ; Titanium dioxide
• 刊名：Journal of Nanoparticle Research
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：16
• 期：1
• 全文大小：
• 作者单位：Claudia Strobel (1)
  Adriano A. Torrano (2)
  Rudolf Herrmann (3)
  Marcelina Malissek (4)
  Christoph Bruchle (2)
  Armin Reller (3)
  Lennart Treuel (4) (5) (6)
  Ingrid Hilger (1)
  
  1. Department of Experimental Radiology, Institute of Diagnostic and Interventional Radiology I, Jena University Hospital-Friedrich Schiller University Jena, Erlanger Allee 101, 07747, Jena, Germany
  2. Department of Chemistry and Center for NanoScience (CeNS), University of Munich (LMU), Butenandtstrae 5-13 (E), 81377, Munich, Germany
  3. Department of Physics, University of Augsburg, Universitaetsstrae 1, 86159, Augsburg, Germany
  4. Physical Chemistry, University of Duisburg-Essen, Universitaetsstrae 5-7, 45117, Essen, Germany
  5. Institute of Applied Physics and Center for Functional Nanostructures (CFN), Karlsruhe Institute of Technology (KIT), 76128, Karlsruhe, Germany
  6. Institut f Mikrotechnik Mainz GmbH, Carl-Zeiss-Str. 18-20, 55129, Mainz, Germany
  
• ISSN：1572-896X

文摘

Until now, the potential effects of titanium dioxide (TiO2) nanoparticles on endothelial cells are not well understood, despite their already wide usage. Therefore, the present work characterizes six TiO2 nanoparticle samples in the size range of 1917 to 8713nm, which are commonly present in sun protection agents with respect to their physicochemical properties (size, shape, potential, agglomeration, sedimentation, surface coating, and surface area), their interactions with serum proteins and biological impact on human microvascular endothelial cells (relative cellular dehydrogenase activity, adenosine triphosphate content, and monocyte chemoattractant protein-1 release). We observed no association of nanoparticle morphology with the agglomeration and sedimentation behavior and no variations of the potential (14 to 19mV) in dependence on the surface coating. In general, the impact on endothelial cells was low and only detectable at concentrations of 100/ml. Particles containing a rutile core and having rod-like shape had a stronger effect on cell metabolism than those with anatase core and elliptical shape (relative cellular dehydrogenase activity after 72h: 60 vs. 90%). Besides the morphology, the nanoparticle shell constitution was found to influence the metabolic activity of the cells. Upon cellular uptake, the nanoparticles were localized perinuclearly. Considering that in the in vivo situation endothelial cells would come in contact with considerably lower nanoparticle amounts than the lowest-observable adverse effects level (100/ml), TiO2 nanoparticles can be considered as rather harmless to humans under the investigated conditions.