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Expression of parathyroid hormone/parathyroid hormone-related peptide receptor 1 in normal and diseased bladder detrusor muscles: a clinico-pathological study
- 作者:Nobuyuki Nishikawa (1) (2)
Rie Yago (3) Yuichiro Yamazaki (4) Hiromitsu Negoro (1) Mari Suzuki (3) Masaaki Imamura (1) (5) Yoshinobu Toda (6) Kazunari Tanabe (3) Osamu Ogawa (1) Akihiro Kanematsu (7)
1. Department of Urology ; Kyoto University Graduate School of Medicine ; Kyoto ; Japan 2. Department of Cell Physiology ; Nagoya City University Graduate School of Medical Sciences ; Nagoya ; Japan 3. Department of Urology ; Tokyo Women’s Medical University ; Tokyo ; Japan 4. Department of Urology ; Kanagawa Children’s Medical Centre ; Yokohama ; Japan 5. Department of Urology ; Otsu Red Cross Hospital ; Otsu ; Japan 6. Department of Clinical Laboratory Science ; Tenri Health Care University ; Tenri ; Japan 7. Department of Urology ; Hyogo College of Medicine ; Nishinomiya ; Japan
- 关键词:Parathyroid hormone ; related peptide ; Parathyroid hormone 1 receptor ; Bladder compliance ; Smooth muscle
- 刊名:BMC Urology
- 出版年:2015
- 出版时间:December 2015
- 年:2015
- 卷:15
- 期:1
- 全文大小:57 KB
- 参考文献:1. Ozkan B, Demirkesen O, Durak H, Uygun N, Ismailoglu V, Cetinel B: Which factors predict upper urinary tract deterioration in overactive neurogenic bladder dysfunction? / Urology 2005,66(1):99-04. 10.1016/j.urology.2005.02.009 CrossRef
2. Stein R, Schroder A, Thuroff JW: Bladder augmentation and urinary diversion in patients with neurogenic bladder: surgical considerations. / J Pediatr Urol 2012,8(2):153-1. 10.1016/j.jpurol.2011.11.014 CrossRef 3. Stein R, Schroder A, Thuroff JW: Bladder augmentation and urinary diversion in patients with neurogenic bladder: non-surgical considerations. / J Pediatr Urol 2012,8(2):145-2. 10.1016/j.jpurol.2011.03.015 CrossRef 4. Park WH: Management of low complaint bladder in spinal cord injured patients. / LUTS 2010,2(2):61-0. 5. Suva LJ, Winslow GA, Wettenhall RE, Hammonds RG, Moseley JM, Diefenbach-Jagger H, / et al.: A parathyroid hormone-related protein implicated in malignant hypercalcemia: cloning and expression. / Science 1987,237(4817):893-. 10.1126/science.3616618 CrossRef 6. Urena P, Kong XF, Abou-Samra AB, Juppner H, Kronenberg HM, Potts JT Jr, / et al.: Parathyroid hormone (PTH)/PTH-related peptide receptor messenger ribonucleic acids are widely distributed in rat tissues. / Endocrinology 1993,133(2):617-3. 7. Philbrick WM, Wysolmerski JJ, Galbraith S, Holt E, Orloff JJ, Yang KH, / et al.: Defining the roles of parathyroid hormone-related protein in normal physiology. / Physiol Rev 1996,76(1):127-3. 8. Nishikawa N, Kanematsu A, Negoro H, Imamura M, Sugino Y, Okinami T, / et al.: PTHrP is endogenous relaxant for spontaneous smooth muscle contraction in urinary bladder of female rat. / Endocrinology 2013,154(6):2058-8. 10.1210/en.2012-2142 CrossRef 9. Hamano S, Yamanishi T, Igarashi T, Murakami S, Ito H: Evaluation of functional bladder capacity in Japanese children. / Int J Urol 1999,6(5):226-. 10.1046/j.1442-2042.1999.00049.x CrossRef 10. Toda Y, Kono K, Abiru H, Kokuryo K, Endo M, Yaegashi H, / et al.: Application of tyramide signal amplification system to immunohistochemistry: a potent method to localize antigens that are not detectable by ordinary method. / Pathol Int 1999,49(5):479-3. 10.1046/j.1440-1827.1999.00875.x CrossRef 11. Lupp A, Klenk C, Rocken C, Evert M, Mawrin C, Schulz S: Immunohistochemical identification of the PTHR1 parathyroid hormone receptor in normal and neoplastic human tissues. / Eur J Endocrinol 2010,162(5):979-6. 10.1530/EJE-09-0821 CrossRef 12. Yamamoto M, Harm SC, Grasser WA, Thiede MA: Parathyroid hormone-related protein in the rat urinary bladder: a smooth muscle relaxant produced locally in response to mechanical stretch. / Proc Natl Acad Sci U S A 1992,- 刊物主题:Urology/Andrology; Internal Medicine;
- 出版者:BioMed Central
- ISSN:1471-2490
文摘
Background To investigate the expression of parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor 1 (PTH1R) in clinical specimens of normal and diseased bladders. PTHrP is a unique stretch-induced endogenous detrusor relaxant that functions via PTH1R. We hypothesized that suppression of this axis could be involved in the pathogenesis of bladder disease. Methods PTH1R expression in clinical samples was examined by immunohistochemistry. Normal kidney tissue from a patient with renal cancer and bladder specimens from patients undergoing ureteral reimplantation for vesicoureteral reflux or partial cystectomy for urachal cyst were examined as normal control organs. These were compared with 13 diseased bladder specimens from patients undergoing bladder augmentation. The augmentation patients ranged from 8 to 31?years old (median 15?years), including 9 males and 4 females. Seven patients had spinal disorders, 3 had posterior urethral valves and 3 non-neurogenic neurogenic bladders (Hinman syndrome). Results Renal tubules, detrusor muscle and blood vessels in normal control bladders stained positive for PTH1R. According to preoperative urodynamic studies of augmentation patients, the median percent bladder capacity compared with the age-standard was 43.6% (range 1.5-6.6%), median intravesical pressure at maximal capacity was 30 cmH2O (range 10-07 cmH2O), and median compliance was 3.93 ml/cmH2O (range 0.05-0.3?ml/cmH2O). Detrusor overactivity was observed in five cases (38.5%). All augmented bladders showed negative stainings in PTH1R expression in the detrusor tissue, but positive staining of blood vessels in majority of the cases. Conclusions Downregulation of PTH1R may be involved in the pathogenesis of human end-stage bladder disease requiring augmentation.
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