Population stock structure of leatherback turtles (Dermochelys coriacea) in the Atlantic revealed using mtDNA and microsatellite markers

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• 作者：Peter H. Dutton (1)
  Suzanne E. Roden (1)
  Kelly R. Stewart (1)
  Erin LaCasella (1)
  Manjula Tiwari (1)
  Angela Formia (2) (3)
  Joao Carlos Thomé (4)
  Suzanne R. Livingstone (5)
  Scott Eckert (6)
  Didiher Chacon-Chaverri (7)
  Philippe Rivalan (8)
  Phil Allman (9)
  
• 关键词：Sea turtle ; Dermochelys coriacea ; Conservation genetics ; Mitochondrial DNA ; Demographically independent populations ; Management ; Recovery plan ; Microsatellites
• 刊名：Conservation Genetics
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：14
• 期：3
• 页码：625-636
• 全文大小：340KB
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• 作者单位：Peter H. Dutton (1)
  Suzanne E. Roden (1)
  Kelly R. Stewart (1)
  Erin LaCasella (1)
  Manjula Tiwari (1)
  Angela Formia (2) (3)
  Joao Carlos Thomé (4)
  Suzanne R. Livingstone (5)
  Scott Eckert (6)
  Didiher Chacon-Chaverri (7)
  Philippe Rivalan (8)
  Phil Allman (9)
  
  1. Protected Resources Division, Southwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, 8901 La Jolla Shores Drive, La Jolla, CA, 92037, USA
  2. Molecular Ecology Research Group, Department of Animal Biology and Genetics, University of Florence, Via Romana 17, 50125, Florence, Italy
  3. Wildlife Conservation Society, Global Conservation Program, 2300 Southern Boulevard, Bronx, NY, 10460, USA
  4. Instituto Chico Mendes de Conservacao da Biodiversidade, Centro Tamar-DIBIO, Av. Paulino Muller, 1111, Vitória, ES, CEP 29.040-715, Brazil
  5. College of Medical, Veterinary & Life Sciences, Institute of Biodiversity, Animal Health & Comparative Medicine, University of Glasgow, Glasgow, G12 8QQ, UK
  6. Biology and Natural Resources Department, Principia College, Elsah, IL, 62028, USA
  7. WIDECAST-Costa Rica, Tibás, San Jose, 496-1100, Costa Rica
  8. Laboratoire d’Ecologie, Systématique et Evolution, Université Paris-XI Orsay, Rue Georges Clé menceau, Orsay, 91405, France
  9. Department of Biological Studies, Florida Gulf Coast University, 10501 Boulevard South, Fort Myers, FL, 33965, USA
  
• ISSN：1572-9737

文摘

This study presents a comprehensive genetic analysis of stock structure for leatherback turtles (Dermochelys coriacea), combining 17 microsatellite loci and 763?bp of the mtDNA control region. Recently discovered eastern Atlantic nesting populations of this critically endangered species were absent in a previous survey that found little ocean-wide mtDNA variation. We added rookeries in West Africa and Brazil and generated longer sequences for previously analyzed samples. A total of 1,417 individuals were sampled from nine nesting sites in the Atlantic and SW Indian Ocean. We detected additional mtDNA variation with the longer sequences, identifying ten polymorphic sites that resolved a total of ten haplotypes, including three new variants of haplotypes previously described by shorter sequences. Population differentiation was substantial between all but two adjacent rookery pairs, and F ST values ranged from 0.034 to 0.676 and 0.004 to 0.205 for mtDNA and microsatellite data respectively, suggesting that male-mediated gene flow is not as widespread as previously assumed. We detected weak (F ST?=?0.008 and 0.006) but significant differentiation with microsatellites between the two population pairs that were indistinguishable with mtDNA data. POWSIM analysis showed that our mtDNA marker had very low statistical power to detect weak structure (F ST?<?0.005), while our microsatellite marker array had high power. We conclude that the weak differentiation detected with microsatellites reflects a fine scale level of demographic independence that warrants recognition, and that all nine of the nesting colonies should be considered as demographically independent populations for conservation. Our findings illustrate the importance of evaluating the power of specific genetic markers to detect structure in order to correctly identify the appropriate population units to conserve.