The Arabidopsis ROP-activated receptor-like cytoplasmic kinase RLCK VI_A3 is involved in control of basal resistance to powdery mildew and trichome branching

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• 作者：Tina Reiner ; Caroline Hoefle ; Christina Huesmann ; Dalma Ménesi…
• 关键词：Receptor ; like cytoplasmic kinase (RLCK) ; RAC/ROP GTPase ; Arabidopsis thaliana ; Erysiphe cruciferarum ; Trichome
• 刊名：Plant Cell Reports
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：34
• 期：3
• 页码：457-468
• 全文大小：1,014 KB
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• 作者单位：Tina Reiner (1)
  Caroline Hoefle (1)
  Christina Huesmann (1)
  Dalma Ménesi (2)
  Attila Fehér (2)
  Ralph Hückelhoven (1)
  
  1. Lehrstuhl für Phytopathologie, Technische Universit?t München, Emil-Ramann Stra?e 2, 85350, Freising-Weihenstephan, Germany
  2. Laboratory of Functional Cell Biology, Biological Research Centre, Institute of Plant Biology, Hungarian Academy of Sciences, P.O. Box 521, Temesvári krt. 62, 6726, Szeged, Hungary
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Sciences
  Biotechnology
  Plant Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-203X

文摘

Key message The Arabidopsis receptor-like cytoplasmic kinase AtRLCK VI_A3 is activated by AtROPs and is involved in trichome branching and pathogen interaction. Abstract Receptor-like cytoplasmic kinases (RLCKs) belong to the large superfamily of receptor-like kinases, which are involved in a variety of cellular processes like plant growth, development and immune responses. Recent studies suggest that RLCKs of the VI_A subfamily are possible downstream effectors of the small monomeric G proteins of the plant-specific Rho family, called ‘Rho of plants-(RAC/ROPs). Here, we describe Arabidopsis thaliana AtRLCK VI_A3 as a molecular interactor of AtROPs. In Arabidopsis epidermal cells, transient co-expression of plasma membrane located constitutively activated (CA) AtROP4 or CA AtROP6 resulting in the recruitment of green fluorescent protein-tagged AtRLCK VI_A3 to the cell periphery. Intrinsic kinase activity of AtRLCK VI_A3 was enhanced in the presence of CA AtROP6 in vitro and further suggested a functional interaction between the proteins. In the interaction of the biotrophic powdery mildew fungus Erysiphe cruciferarum (E. cruciferarum) and its host plant Arabidopsis, Atrlck VI_A3 mutant lines supported enhanced fungal reproduction. Furthermore Atrlck VI_A3 mutant lines showed slightly reduced size and an increase in trichome branch number compared to wild-type plants. In summary, our data suggest a role of the AtROP-regulated AtRLCK VI_A3 in basal resistance to E. cruciferarum as well as in plant growth and cellular differentiation during trichome morphogenesis. Results are discussed in the context of literature suggesting a function of RAC/ROPs in both resistance and susceptibility to pathogen infection.